Multiplex PCR assay for direct identification of group B streptococcal alpha-protein-like protein genes |
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| Authors: | Creti Roberta Fabretti Francesca Orefici Graziella von Hunolstein Christina |
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| Affiliation: | Laboratorio di Batteriologia, Istituto Superiore di Sanità, 00161 Rome, Italy. roberta.creti@iss.it |
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| Abstract: | We developed a group B streptococcus multiplex PCR assay which allows, by direct analysis of the amplicon size, determination of the surface protein antigen genes of alpha-C protein, epsilon protein, Rib, Alp2, Alp3, and Alp4. The multiplex PCR assay offers a rapid and simple method of subtyping Streptococcus agalactiae based on surface protein genes. |
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