不同种植体表面改性对细菌生物膜的影响

目的:研究临床常用的3种钛种植体不同表面处理对口腔内常见菌群牙龈卟啉单胞菌(Porphyromanusgingivalis,P.gingivalis)、具核梭杆菌(Fusobacterium nucleatum,F.nucleatum)及血链球菌(Streptococcus sanguis,S.sanguis)生物膜黏附的影响。方法:将钛片分成3组进行表面改性处理,分别为:电解沉积羟基磷灰石(HA)组、微弧氧化(MAO)组和大颗粒喷砂酸蚀(SLA)组,进行表面性能检测;在3组钛片上分别培养Pg、Fn及Ss 4 d,通过菌落形成单位(CFU)法、噻唑蓝(MTT)法和扫描电子显微镜(SEM)探究细菌的黏附情况。结果:(1)SEM观察表面形貌可见HA组表面大量不规则的棒状结构;MAO表面“火山口”样结构,孔径在0.1~1μm之间;SLA表面为不规则凹陷,大小不一且边缘锋利。3组表面粗糙度Ra值:SLA组0.05);Pg生物膜在MAO组钛片上活性最高,与另外2组有统计学差异(P<0.01)。(3)利用SEM观察显示,细菌活性与种植体表面形态结构有关。结论:细菌在种植体表面黏附受到表面形貌影响,与粗糙度及物理构型有关。对于Pg、Ss和Fn 3种细菌,SLA在3种表面处理中细菌粘附量最少。

Effect of Surface Modification of Different Implants on Bacterial Biofilm

Objective:To study the effects of surface treatments commonly used in clinic on the adhesion of Porphyroman gingivalis(Pg),Fusobacterium nucleatum(Fn),and Streptococcus sanguis(Ss).Methods:Titanium discs were divided into three groups for surface modification:electrodeposited hydroxyapatite(HA)group,micro-arc oxidation(MAO)group,and sandblasted with large-grit and acid-etched(SLA)group.The surface properties were tested.Fn,Ss,and Pg were cultured on three groups of titanium discs for 4 days,respectively.The colony forming unit(CFU)method,thiazole blue(MTT)method,and scanning electron microscopy(SEM)were used to observe bacterial adhesion.Results:(1)SEM observation showed that there were a large number of irregular rod-like structures on the surface of HA group,crater-like structures on the surface of MAO with aperture ranging from 0.1 to 1 micron,and irregular depressions on the surface of SLA with different sizes and sharp edges.Ra values of surface roughness in three groups was:SLA group0.05).Pg biofilm had the highest activity on titanium plate in MAO group,with statistical difference from the other two groups(P<0.01).(3)SEM observation showed that the bacterial activity was related to the morphological structure of implant surface.Conclusion:The adhesion of bacteria to implant surface is affected by surface morphology,which is related to roughness and physical configuration.For three kinds of bacteria,SLA had the least bacterial adhesion.