3D打印制备不同重量比例的n-HA/PLA/PVA复合膜性能比较

目的:通过3D打印的方法制备的不同重量比例的纳米羟基磷灰石/聚乳酸/聚乙烯醇(n-HA/PLA/PVA)复合膜,并对其相关性能检测。方法:采用3D打印技术制备不同重量比例的n-HA/PLA/PVA复合膜,分别为PLA/PVA复合膜、15%n-HA/PLA/PVA复合膜、50%n-HA/PLA/PVA复合膜、75%n-HA/PLA/PVA复合膜。扫描电镜下观察各组膜形态,对其力学性能、细胞毒性及动物实验相应指标进行检测。结果:扫描电镜下观察,n-HA/PLA/PVA复合膜呈现三维网状结构,各材料间相互结合,孔隙分布不均,大小不一。随着n-HA质量浓度的提高,电镜下见各材料间孔隙逐渐减小,形成结构均匀的复合膜。力学性能及吸水率检测中,随着nHA含量的增加,n-HA/PLA/PVA复合膜的拉伸强度及吸水率呈下降趋势;细胞毒性检测,不同比例复合膜的细胞增殖率无明显差别,无细胞毒性。动物实验测量牙周菌斑指数及龈沟出血指数未发现不同比例n-HA/PLA/PVA复合膜有统计学差异。结论:3D打印不同比例的n-HA/PLA/PVA复合膜具有良好的物理性能和细胞生物相容性,n-HA比例更高的复合膜可能具有更好的物理性能及良好的生物相容性。

Comparison of n-HA/PLA/PVA Composite Membranes Prepared by 3D Printing with Different Weight Ratios

Objective:To prepare and test nano-hydroxyapatite/polylactic acid/polyvinyl alcohol(n-HA/PLA/PVA)composite membranes with different weight ratios.Methods:3D printing technology was used to prepare n-HA/PLA/PVA composite membranes with different weight ratios,namely PLA/PVA composite membrane,15%n-HA/PLA/PVA composite membrane,50%n-HA/PLA/PVA composite membrane,and 75%n-HA/PLA/PVA composite membrane.The membrane morphology was observed with scanning electron microscope(SEM),the mechanical properties,cytotoxicity,and corresponding indices in animal experiments were detected.Results:SEM showed that the n-HA/PLA/PVA composite membrane exhibited a three-dimensional network structure,the materials were combined with each other,the distribution of pore was uneven,and the size was different.With the increase of the mass concentration of n-HA,the porosity between the materials was gradually reduced,and a composite membrane with uniform structure was formed.In the test of mechanical properties and water absorption,the tensile strength and water absorption of n-HA/PLA/PVA composite membranes decreased with the increase of n-HA.The cytotoxicity test showed no cytotoxicity and no significant difference in the cell proliferation rate of different proportions of composite membranes.It was found that there was no statistically difference in the ratios of n-HA/PLA/PVA composite membranes by measured periodontal plaque index and sulcus bleeding index in animal experiments.Conclusion:3D printing n-HA/PLA/PVA composite membranes with different ratios have good physical properties and cell biocompatibility.Composite membranes with higher n-HA ratio may have better physical properties and biocompatibility.