Shh蛋白与慢性牙周炎炎症程度的相关性研究

目的:探讨Sonic Hedgehog(Shh)信号通路中Shh蛋白与慢性牙周炎炎症程度的相关性。方法:选择健康对照20例(健康对照组),轻度牙周炎患者20例(轻度牙周炎组),中重度牙周炎患者20例(中重度牙周炎组),收集其龈沟液样本。采用ELISA法检测龈沟液中Shh蛋白、白细胞介素-6(IL-6)及白细胞介素-10(IL-10)的水平。结果:Shh蛋白、IL-6在慢性牙周炎组的水平均高于健康对照组(P<0.05),且中重度慢性牙周炎组高于轻度慢性牙周炎组(P<0.05);IL-10在轻度慢性牙周炎组的水平高于健康对照组及中重度慢性牙周炎组(P<0.05),且健康对照组高于中重度慢性牙周炎组(P<0.05);Shh蛋白、IL-6水平与出血指数(BI)、探诊深度(PD)均呈正相关(P<0.05)。结论:Shh信号通路可能参与了慢性牙周炎的炎症反应过程。

Correlation between Shh Protein and Inflammatory Degree of Chronic Periodontitis

Objective:To investigate the correlation between Sonic Hedgehog(Shh)protein and inflammatory degree of chronic periodontitis in Shh signaling pathway.Methods:The gingival crevicular fluid samples were collected from 20 healthy controls,20 patients with mild periodontitis,and 20 patients with moderate to severe periodontitis.Gingival crevicular fluid level of Shh protein,IL-6,and IL-10 were measured by enzyme linked immunosorbent assay(ELISA).Results:The levels of Shh protein and IL-6 in gingival crevicular fluid were higher in chronic periodontitis group than those in healthy control group(P<0.05),and in moderate and severe chronic periodontitis group were higher than those in mild chronic periodontitis group(P<0.05).The level of IL-10 in mild chronic periodontitis group was higher than that in healthy control group and moderate-severe chronic periodontitis group(P<0.05),and that in healthy control group was higher than that in moderate-severe chronic periodontitis group(P<0.05).The levels of Shh protein and IL-6 were positively correlated with BI and PD(P<0.05).Conclusion:Ssh signaling pathway may be involved in the inflammatory response of chronic periodontitis.