柚皮苷对脂多糖诱导的人牙周膜成纤维细胞增殖及炎症因子表达的影响

目的探讨柚皮苷(naringin,NRG)对脂多糖诱导的人牙周膜成纤维细胞增殖及炎症因子表达的影响。方法以脂多糖(lipopolysaccharide,LPS)(100μg/mL)诱导人牙周膜成纤维细胞(human periodontal ligament fibroblast,HPDLF)损伤,采用MTT法检测NRG对LPS诱导HPDLF损伤的保护作用,利用ELISA法和Western blotting法检测NRG对LPS诱导的HPDLF中白介素(interleukin,IL)-6、IL-8、IL-1β等炎症因子蛋白表达的影响,同时利用Real-time PCR方法检测NRG对LPS诱导的HPDLF组IL-6、IL-8、IL-1β等炎症因子基因表达的影响。结果经LPS(100μg/mL)刺激后,HPDLF增殖受到抑制,与CON组比较,LPS组细胞存活率显著降低,细胞周期进程受到抑制,差异具有统计学意义(P<0.05);不同浓度的NRG(40,20,10μg/mL)可显著抑制LPS诱导的HPDLF损伤,并可降低细胞内炎症因子表达;与LSP组比较,LPS+NRG 40μg/mL组、LPS+NRG 20μg/mL组、LPS+NRG 10μg/mL组HPDLF存活率显著提升,细胞周期进程加快,抑制IL-6、IL-8、IL-1β蛋白及基因表达水平均显著降低,差异具有统计学意义(P<0.05)。结论NRG对LPS诱导的HPDLF损伤具有保护作用,并可通过抑制LPS诱导的HPDLF中IL-6、IL-8、IL-1β的蛋白及基因表达水平,促进HPDLF增殖。

Effects of naringin on proliferation and expression of inflammatory cytokines in human periodontal ligament fibroblast induced by lipopolysaccharide

Objective To investigate the effects of naringin(NRG)on the proliferation and expression of inflammatory factors of human periodontal ligament fibroblast(HPDLF)induced by lipopolysaccharide(LPS).Methods HPDLF was induced by lipopolysaccharide(LPS)(100μg/mL).The protective effect of NRG on LPS induced HPDLF injury was detected by MTT assay.The effects of NRG on the expression of IL-6,IL-8,IL-1βand other inflammatory factors in LPS-induced HPDLF were detected by ELISA and Western blotting.The effects of NRG on the expression of IL-6,IL-8 and IL-1βin LPS-induced HPDLF were also detected by Real-time PCR.Results The proliferation of HPDLF was inhibited after stimulation with LPS(100μg/mL).Compared with the CON group,the cell survival rate of LPS group significantly reduced,and the cell cycle process was inhibited.The difference was statistically significant(P<0.05).Different concentrations of NRG(40,20,10μg/mL)could significantly inhibit LPS-induced HPDLF injury and reduce the expression of inflammatory factors in cells(P<0.05).Compared with LSP group,the survival rates of HPDLF in LPS+NRG 40μg/mL group,LPS+NRG 20μg/mL group,LPS+NRG 10μg/mL group increased significantly,the cell cycle process accelerated,and the levels of inhibiting IL-6,IL-8,IL-1βprotein and gene expression decreased significantly.There was significant difference(P<0.05).Conclusion NRG has protective effect on LPS-induced HPDLF injury,and can promote the proliferation of HPDLF cells by inhibiting the expression levels of the protein and genes of IL-6,IL-8,IL-1βin LPS-induced HPDLF.