5周间歇性负重游泳训练对大鼠睾丸Leydig细胞胆固醇代谢的影响

采用实时荧光定量PCR的Taqman技术探讨5周间歇性负重游泳训练导致大鼠血清睾酮水平降低时,大鼠睾丸Leydig细胞胆固醇代谢关键环节上的HMG-CoA还原酶、LDL-R、SR-BI、类固醇激素合成急性调控蛋白(StAR)基因表达的变化。结果显示,运动引起血清睾酮显著降低时(P<0.01),Leydig细胞内LDL-R mRNA表达量显著增加(P<0.05),HMG-CoA还原酶、SR-BI、StAR mRNA表达量无显著性变化。结果表明当大鼠以负重5%体重的负荷强度进行5周的间歇性游泳训练引起血清睾酮显著降低时,Leydig细胞通过LDL-R介导的内吞作用摄取外源性胆固醇作用加强,Leydig细胞内胆固醇的合成、摄取和转运的关键环节未发生障碍。

Effects of Five-week Intermittent Swimming on the Cholesterol Metabolism in Leydig Cells of Rat

The gene expressions of HMG-CoA reductase,LDL-R,SR-BI and StAR in Leydig cells of rats forced to swim with the load of 5% body weight for 5 weeks were detected using the Taqman technique of real-time fluorescence quantitative PCR.With the significantly decreasing of serum testosterone caused by the swimming,the mRNA level of LDL-R increase significantly and the gene expression of HMG-CoA reductase,SR-BI and StAR in Leydig cells remain unchanged.The result suggests that when serum testosterone decrease appears after five-weeks intermittent swimming with the load of 5% body weight,the ability to intake cholesterol is strengthened firstly by the encytosis of LDLR in Leydig cells,and the rats with lower serum testosterone have no trouble in the key processes of the cholesterol biosynthesis,intaking and transporting in Leydig cells.