桃红四物汤促进产后血瘀大鼠子宫修复的作用

目的 观察桃红四物汤对产后血瘀大鼠子宫修复的促进作用，探讨其与内皮素-1（endothelin-1，ET-1）、诱导型一氧化氮合酶（inducible nitric oxide synthase，iNOS）及血管内皮生长因子（vascular endothelial growth factor，VEGF）和血管内皮生长因子受体2（vascular endothelial growth factor receptor 2，VEGFR-2）的关联性。方法 选取产后血瘀模型复制成功的大鼠40只，将其随机分为桃红四物汤高剂量（18.0 g/kg）、中剂量（9.0 g/kg）、低剂量（4.5 g/kg）组，阳性对照组（益母草颗粒4.3 g/kg）和模型组（蒸馏水），另取正常雌性大鼠作为正常对照组。连续给药7 d后，腹主动脉取血，分离子宫，苏木精-伊红染色法观察子宫血瘀状态，免疫组织化学法检测子宫VEGFR-2表达情况；酶联免疫吸附法检测血清中ET-1、iNOS及VEGF水平。结果 桃红四物汤可明显改善子宫血瘀程度，促进子宫组织中VEGFR-2的表达；桃红四物汤高、中剂量组大鼠血清VEGF及iNOS水平明显升高（P＜0.05），ET-1水平明显降低（P＜0.05）。结论 桃红四物汤可促进产后血瘀大鼠子宫修复，其修复作用可能与调节子宫血管舒缩功能及促进子宫血管新生有关。

Efficacy of Taohong Siwu Decoction in Promoting Uterine Repair in Rats with Postpartum Blood Stasis

Objective To observe the efficacy of Taohong Siwu Decoction in promoting uterine repair in rats with postpartum blood stasis, and to investigate its effects on endothelin-1 (ET-1), inducible nitric oxide synthase (iNOS), vascular endothelial growth factor (VEGF), and vascular endothelial growth factor receptor 2 (VEGFR-2). Methods Forty rats in which a model of postpartum blood stasis was successfully established were randomly divided into high-, middle-, and low-dose Taohong Siwu groups (18.0 g/kg, 9.0 g/kg, and 4.5 g/kg), positive control group (Herba Leonuri Granule 4.3 g/kg), and model group (distilled water); normal female rats were selected as normal control group. All rats received respective treatments for 7 consecutive days. Blood was collected from the abdominal aorta, and the uterus was isolated. HE staining was used to evaluate blood stasis in the uterus. The expression of VEGFR-2 in the uterus was measured by immunohistochemistry. Serum levels of ET-1, iNOS, and VEGF were determined by ELISA. Results Taohong Siwu Decoction significantly relieved blood stasis in the uterus and increased the expression of VEGFR-2 in the uterus. The high- and middle-dose Taohong Siwu Decoction groups had significantly increased serum levels of VEGF and iNOS (P<0.05) and a significantly reduced serum level of ET-1 (P<0.05). Conclusion Taohong Siwu Decoction can promote uterine repair in rats with postpartum blood stasis, possibly by regulating uterine vasomotion and promoting uterine angiogenesis.