| 贝母素甲抑制人乳腺癌细胞MCF-7/TAM增殖及其对细胞凋亡的影响 |
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| 引用本文： | 谌海燕,陈信义. 贝母素甲抑制人乳腺癌细胞MCF-7/TAM增殖及其对细胞凋亡的影响[J]. 中医药学报, 2012, 40(4): 12-15 |
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| 作者姓名： | 谌海燕 陈信义 |
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| 作者单位： | 1. 北京中医药大学东直门医院,北京,100700
2. 北京中医药大学东直门医院,北京100700;北京市与教育部重点实验室,北京100700 |
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| 基金项目： | 北京中医药大学自主创新课题（2009TYBZZ-XS057） |
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| 摘 要： | 目的:探讨贝母素甲抑制耐三苯氧胺人乳腺癌细胞MCF-7/TAM的增殖及其机制。方法:采用四甲基偶氮唑蓝(MTT)比色分析法检测贝母素甲作用于MCF-7/TAM细胞后的抑制率;用流式细胞术检测细胞周期和凋亡率;用免疫细胞化学法检测Bcl-2表达变化。结果:贝母素甲对MCF-7/TAM细胞有明显的抑制作用,呈浓度和时间依赖性。作用48h后能诱导细胞凋亡,细胞周期被阻滞于G1期(P0.05)。免疫细胞化学法检测Bcl-2表达减弱(P0.01)。结论:贝母素甲可抑制MCF-7/TAM细胞的增殖,并诱导其凋亡。
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| 关 键 词： | 乳腺癌 贝母素甲 细胞凋亡 细胞周期 |
Effect of Peimine on Inhibiting the MCF -7/TAM Proliferation of Human Breast Cancer Cell and Its Influence on Cell Apoptosis |
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| CHEN Hai-yan , CHEN Xin-yi. Effect of Peimine on Inhibiting the MCF -7/TAM Proliferation of Human Breast Cancer Cell and Its Influence on Cell Apoptosis[J]. Acta Chinese Medicine and Pharmacology, 2012, 40(4): 12-15 |
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| Authors: | CHEN Hai-yan CHEN Xin-yi |
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| Affiliation: | CHEN Hai -yan1 ,CHEN Xin - yi1,2 (1. Dongzhimen Hospital, Beifing University of Chinese Medicine, Beifing 100700, China; 2. The Key Laboratory of Beijing and Ministry of Education Beijing 100700, China) |
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| Abstract: | Objective： To investigate the inhibit proliferation and its mechanism of peimine on human breast cancer cells resistant to tamoxifen （ MCF - 7/TAM）. Methods ： Detect inhibitive rate under the influence of peimine on MCF - 7/TAM cells by the method of tetrazolium blue（MTT） colorimetric assay, detect cell cycle,apoptotic rate by flow cytometry and detect changes in the expression of Bcl -2 by immunocytochemistry. Results： Peimine had a significant inhibition on MCF - 7/TAM cells, which depended on concentration and time. It could induce apoptosis after 48 hours. The cell cycle was arrested at G1 phase （ P 〈 0.05 ）. It was detected by immunocytochemistry that the expression of Bcl - 2 decreased （ P 〈 0.01 ）. Conclusion ： Peimine can inhibit the proliferation of MCF - 7/TAM cells and induce its apoptosis. |
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| Keywords: | Breast cancer Peimine Apoptosis Cell cycle |
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