脊髓背角P物质在电针调控炎性痛大鼠神经-免疫网络中的作用研究

目的:通过阐明在脊髓背角疼痛信号传输中的关键神经肽SP在电针夹脊穴治疗大鼠单发关节炎中的作用,揭示夹脊电针抑制炎性痛的神经-免疫机制。方法:随机数字表法将大鼠分为假造模组、模型组、西乐葆组、电针组、电针+西乐葆组,采用弗氏完全佐剂建立的单侧足底慢性炎症痛大鼠模型,采用实时定量PCR法检测大鼠脊髓中SP mRNA在电针夹脊穴治疗大鼠单发关节炎中过程中的变化。结果:各组单侧足底慢性炎症痛大鼠模型脊髓中的SP mRNA表达与假造模组比较显著性增加(P0.001);电针夹脊穴组大鼠SP mRNA的表达与模型组比较明显降低(P0.01),且电针夹脊穴增强灌饲西乐葆对炎性痛大鼠脊髓中SP mRNA表达的抑制作用(P0.01)。结论:电针通过下调炎性痛大鼠神经-免疫网络环路中的重要神经肽脊髓背角SP的表达,从而发挥镇痛作用。

Involvement of Substance P in Inflammmatory Pain and Electroacupuncture Regulating Network of Immune - neuroendocrine Interactions

Objective ： To reveal the mechanism of involvement of spinal cord pain control system SP in inflammatory pain and the regulation of network of immune - neuroendocrine interactions by electroacupuncture （EA）. Methods ： The rats were randomly divided into sham surgery group, model group, Celebre group, EA group, EA plus Celebre group. Monoar- thritis （MA） was induced by an injection of complete Freund＇ s adjuvant （CFA）. The present study was designed to ex- plore the changes mRNA levels of SP in spinal cord of MA rats and EA analgesia by using Real - time PCR. Results ：The mRNA levels detected by Real - time PCR of SP in spinal cord in EA group were significantly decreaced as compared to those in MA group （ P 〈 0.01 ） ;Combination of EA with Celebre significantly enhanced the inhibitory effects （ P 〈 0.01 ）. Conclusion：Celebre and EA synergistically block activation of spinal microglia and subsequent astrocytes can reduce the release of SP in the spinal cord, thereby leading to a reinforcing inhibitory effect on MA - induced thermal hyperalgesia.