| 丹柴解郁片质量标准的研究 |
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| 引用本文： | 丁大勇,张妍妍,姚琳.丹柴解郁片质量标准的研究[J].中医药学报,2012,40(4):77-80. |
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| 作者姓名： | 丁大勇 张妍妍 姚琳 |
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| 作者单位： | 1. 大庆华科股份有限公司药业分公司,黑龙江大庆,163316
2. 黑龙江省中医研究院,黑龙江哈尔滨,150036 |
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| 摘 要： | 目的:建立丹柴解郁片的质量标准。方法:采用TLC法对处方中白术、牡丹皮、白芍、栀子进行定性鉴别研究;采用HPLC法测定柴胡皂苷a、d的含量,选用Dikma ODS C18(4.6mm×250mm,5μm)色谱柱,乙腈-水(44∶56)为流动相,检测波长210nm,流速1.0mL/min。结果:TLC法可鉴别出白术、牡丹皮、白芍、栀子的特征斑点,且专属性强,分离度好;柴胡皂苷a在45.28～226.4μg/mL(r=0.999 5)范围内、柴胡皂苷d在48.16～240.8μg/mL(r=0.999 3)范围内,浓度与峰面积呈良好的线性关系,平均回收率分别为96.41%(RSD=1.28%)、99.52%(RSD=0.74%)。结论:该方法简便可行,可用于丹柴解郁片的质量控制。
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| 关 键 词： | 丹柴解郁片 TLC 柴胡皂苷a 柴胡皂苷d HPLC |
Quality Standard of Danchai Jieyu Pills |
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| DING Da-yong
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ZHANG Yan-yan
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YAO Lin.Quality Standard of Danchai Jieyu Pills[J].Acta Chinese Medicine and Pharmacology,2012,40(4):77-80. |
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| Authors: | DING Da-yong ZHANG Yan-yan YAO Lin |
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| Institution: | 2 ( i. The Medical Branch Company of Daqing Hake Co. , Ltd, Daqing 163316, China ; 2. Heilongjiang Academy of TCM,Harbin 150036, China) |
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| Abstract: | Objective： To build the quality standard of Danchai Jieyu pills. Methods： Study on the qualitative idcntifiea- tion of Atractylodis Macroeephalae Rhizoma, Moutan Cortex, Paeoniae Radix and Gardeniae Fructus by TLC;Content de- termination of saikosaponin -a and saikosaponin -d by HPLC, Dikma ODS C18 chromatographic column （4.6ram × 250mm, 5 μm） , ACN ： water （44：56 ） as mobile phase, flow rate 1 mL · min^ - 1. Results ： Distinguish the characteristic spots of Danehai Jieyu pills. The method had a highly specific and good resolution. The linear range of saikosaponin - a was 45.28 - 226.4 μg · mL^- 1 （ r = 0. 9995 ） and saikosaponin - d was 48.16 - 240.8 μg · mL^- 1 （ r = 0. 9993 ）. The average recover rate was 96.41% （ RSD = 1.28% ） ,99.52% （ RSD = 0.74% ）. Conclusion ： The method is simple and feasible. It can be used to control the quality of Danehai Jieyu pills. |
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| Keywords: | Danchai Jieyu pills TLC Saikosaponin - a Saikosaponin - d HPLC |
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