| 高效液相色谱法测定骨宁片中淫羊藿苷的含量 |
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| 引用本文： | 时颖,金向群,张彦,张英. 高效液相色谱法测定骨宁片中淫羊藿苷的含量[J]. 延边医学院学报, 2003, 26(2): 104-105 |
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| 作者姓名： | 时颖 金向群 张彦 张英 |
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| 作者单位： | 吉林省武警总队医院 吉林长春130052(时颖)，吉林大学药学院 吉林长春130021(金向群，张彦)，延边药品检验所 吉林延吉133000(张英) |
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| 摘 要： | [目的 ]用高效液相色谱法测定骨宁片中淫羊藿苷的含量 .[方法 ]采用ZoobaxC18(4 6mm×2 5 0 0mm)色谱柱 ,乙腈 水 (体积比为 30∶70 )为流动相 ,检测波长为 2 70nm ,柱温为 4 0℃ ,流速为 1 0mL/min .[结果 ]线性范围为 0 0 80 4～ 0 4 82 4 μg ,线性关系良好 ,回归方程为Y =6 6 75 3 3X +110 2 7,相关系数为r =0 9999,平均回收率为 99 0 % ,RSD为 1 0 % .[结论 ]高效液相色谱法适合于测定骨宁片中淫羊藿苷的含量
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| 关 键 词： | 药,中草 色谱法,高压液相 质量控制 |
| 文章编号： | 1000-1824(2003)02-0104-02 |
| 修稿时间： | 2003-05-12 |
Determination of the shorthorned epimedium herb glycoside in the Gu Ning Tablets by HPLC |
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| SHI Ying ,JIN Xiang qun ,ZHANG Yan ,ZHANG Ying. Determination of the shorthorned epimedium herb glycoside in the Gu Ning Tablets by HPLC[J]. Journal of Medical Science Yanbian University, 2003, 26(2): 104-105 |
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| Authors: | SHI Ying JIN Xiang qun ZHANG Yan ZHANG Ying |
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| Affiliation: | SHI Ying 1,JIN Xiang qun 2,ZHANG Yan 2,ZHANG Ying 3 |
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| Abstract: | OBJECTIVE To determine the content of the shorthorned epimedium herb glycoside in the Gu Ning Tablets by HPLC. ?METHODS?The Zoobax C 18 Column(4 6?mm×250 0?mm)was used, the methyl cyanide water (V∶V=30∶70) mixture as the mobile phase, with the detection at 270?nm, and column temperature was 40?℃, and the flow rate was 1 0?mL/min.?RESULTS?The linear calibration curves are obtained in the concentration ranges of 0 080?4～0 482?4?μg. The regression equation was Y =66?753 3? X + 1?102 7, r =0 999?9. The average recovery was 99 0%, and RSD was 1 0%.?CONCLUSION?The HPLC fit for detecting the content of the shorthorned epimedium herb glycoside in the Gu Ning Tablets. |
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| Keywords: | drugs Chinese herbal chromatography high pressure liquid quality control |
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