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高效液相色谱法测定骨宁片中淫羊藿苷的含量
引用本文:时颖,金向群,张彦,张英. 高效液相色谱法测定骨宁片中淫羊藿苷的含量[J]. 延边医学院学报, 2003, 26(2): 104-105
作者姓名:时颖  金向群  张彦  张英
作者单位:吉林省武警总队医院 吉林长春130052(时颖),吉林大学药学院 吉林长春130021(金向群,张彦),延边药品检验所 吉林延吉133000(张英)
摘    要:[目的 ]用高效液相色谱法测定骨宁片中淫羊藿苷的含量 .[方法 ]采用ZoobaxC18(4 6mm×2 5 0 0mm)色谱柱 ,乙腈 水 (体积比为 30∶70 )为流动相 ,检测波长为 2 70nm ,柱温为 4 0℃ ,流速为 1 0mL/min .[结果 ]线性范围为 0 0 80 4~ 0 4 82 4 μg ,线性关系良好 ,回归方程为Y =6 6 75 3 3X +110 2 7,相关系数为r =0 9999,平均回收率为 99 0 % ,RSD为 1 0 % .[结论 ]高效液相色谱法适合于测定骨宁片中淫羊藿苷的含量

关 键 词:药,中草  色谱法,高压液相  质量控制
文章编号:1000-1824(2003)02-0104-02
修稿时间:2003-05-12

Determination of the shorthorned epimedium herb glycoside in the Gu Ning Tablets by HPLC
SHI Ying ,JIN Xiang qun ,ZHANG Yan ,ZHANG Ying. Determination of the shorthorned epimedium herb glycoside in the Gu Ning Tablets by HPLC[J]. Journal of Medical Science Yanbian University, 2003, 26(2): 104-105
Authors:SHI Ying   JIN Xiang qun   ZHANG Yan   ZHANG Ying
Affiliation:SHI Ying 1,JIN Xiang qun 2,ZHANG Yan 2,ZHANG Ying 3
Abstract:OBJECTIVE To determine the content of the shorthorned epimedium herb glycoside in the Gu Ning Tablets by HPLC. ?METHODS?The Zoobax C 18 Column(4 6?mm×250 0?mm)was used, the methyl cyanide water (V∶V=30∶70) mixture as the mobile phase, with the detection at 270?nm, and column temperature was 40?℃, and the flow rate was 1 0?mL/min.?RESULTS?The linear calibration curves are obtained in the concentration ranges of 0 080?4~0 482?4?μg. The regression equation was Y =66?753 3? X + 1?102 7, r =0 999?9. The average recovery was 99 0%, and RSD was 1 0%.?CONCLUSION?The HPLC fit for detecting the content of the shorthorned epimedium herb glycoside in the Gu Ning Tablets.
Keywords:drugs   Chinese herbal  chromatography   high pressure liquid  quality control
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