S-8型大孔吸附树脂纯化泽兰总黄酮(酚酸)的工艺研究

目的研究S-8型大孔树脂精制泽兰总黄酮（酚酸）的工艺条件。方法以总黄酮（酚酸）、咖啡酸含量结合指纹图谱主要特征峰面积保留率为考察指标;采用紫外-可见分光光度法测定总黄酮（酚酸）含量,HPLC测定咖啡酸含量并分析指纹图谱主要特征峰面积。结果 S-8型大孔吸附树脂纯化泽兰总黄酮（酚酸）的最佳工艺条件为：上样液浓度为含生药量0.1g·mL-1,上样量为每10g干树脂上样7g生药,吸附速率为1mL·min-1,水洗脱量为60mL,洗脱溶媒为80mL70%乙醇,洗脱速率为2mL·min-1;树脂经95%乙醇和1moL·L-1氢氧化钠溶液再生后,至少可重复使用5次。总黄酮（酚酸）与咖啡酸的保留率为75.56%及104.59%,峰1～5的保留率均达89%以上,纯化后总黄酮（酚酸）及咖啡酸的含量分别提高了3.99倍和4.69倍。结论 S-8型大孔吸附树脂能较好地纯化富集泽兰总黄酮（酚酸）。

Study on Purification of the Total Flavonoids (Phenolic Acids) from Herba Lycopi with S-8 Macroporous Adsorption Resin

OBJECTIVE To study the technologic conditions of purifying the total flavonoids (phenolic acids) from Herba Lycopi by S-8 resin. METHODS UV-VIS spectrophotometry was used to determine the total flavonoids (phenolic acids), HPLC was used to determine caffeic acid and analyze the characteristic peaks of fingerprint. RESULTS The results showed that the effect was perfect when the concentration of original solution of the extract of Herba Lycopi was 0.1 g·mL^-1, the loading amount was 7 g Herba Lycopi per 10 g dry S-8 resin, the adsorption velocity was 1 mL·min^-1, the volume of water was 60 mL, the eluant was 80 mL 70% alcohol, and the elution velocity was 2 mL·min^-1. AB-8 resin could be used at least 5 times repeatedly after being reproducted by 95% ethanol and 1 moL·L^-1 NaOH. The remaining rate of the total flavonoids (phenolic acids) and caffeic acid was 75.56% and 104.59%, and the remaining rate of peak 1-5 was over 89%. The content of the total flavonoids and caffeic acid was raised 3.99 times and 4.69 times after being purified by the S-8 resin. CONCLUSION S-8 resin was fit for separating and purifying the total flavonoids (phenolic acids) from Herba Lycopi.