人类重组酸性成纤维细胞生长因子对细胞增殖和凋亡的影响

目的：通过对人类重组型和野生型酸性成纤 维细胞生长因子（raFGF，waFGF）的比较研究，探讨raFGF与促分裂性有关的生物学作用。 方法：应用NIH3T3细胞株，用MTT法通过细胞增殖实验检测waFGF和raFGF 的促分裂性，通过流式细胞术检测细胞凋亡、细胞膜通透性和线粒体膜电位。同时检测肝素 （HS）对aFGF生物学作用的影响。结果：raFGF对细胞增殖 作用明显低于waFGF。raFGF对细胞凋亡的抑制作用及其对细胞膜通透性的增强作用也明显低 于waFGF，对线粒体膜电位的增强作用明显低于waFGF。HS对aFGF的生物学作用具有促进作用 。结论：raFGF的促分裂作用低于waFGF，而对细胞凋亡的影响不明显。

Effects of human recombinant acidic fibroblast growth factor on cell proliferation and apoptosis in NIH3T3 cells

AIM: Through studying the differences between wild-type acidic fibroblast growth factor (waFGF) with recombinant aFGF (raFGF), the biological effect of raFGF concerned with mitogenic activity was evaluated. METHODS: NIH3T3 cell line was used. Cell proliferation with MTT method was used to study the mitogenic activity. Flow cytometry was also used for detection of apoptosis, cell membrane permeability and mitochondria potential. The role of heparin sulfate (HS) on aFGF biological effect was studied at the same time in this research. RESULTS: The enhancement of raFGF on cell proliferation was significantly lower than that of waFGF. The restriction of raFGF on apoptosis and the enhancement of it on cell membrane permeability were all lower than those of waFGF significantly. The enhancement of raFGF on mitochondria potential was lower than that of waFGF significantly. The HS improved the biological effect of aFGF. CONCLUSION: The mitogenic activity of raFGF is lower than that of waFGF and raFGF has little effect on apoptosis.