番泻叶提取物对豚鼠结肠平滑肌细胞的收缩作用（英文）

目的　研究番泻叶提取物对游离的豚鼠结肠平滑肌细胞收缩的影响 .方法用含 1 g· L-1胶原酶及 0 .1 g· L-1大豆胰蛋白酶抑制剂的消化液消化 ,获得游离的单个豚鼠结肠平滑肌细胞 ;将不同浓度番泻叶提取物加入细胞悬液中 ,用测微尺观察随机遇到的 50个细胞长轴变化情况 ,计算用药前后细胞平均长度 .结果　成功获得了游离的结肠平滑肌细胞 .证实不同浓度的番泻叶提取物 (0 .2 2 ,0 .44,0 .89g· L-1 )与平滑肌细胞共孵育后 ,可使细胞的平均长度缩短为 (93±2 0 ) ,(86± 2 5) ,(85± 1 5)μm,生理盐水对照组为 (1 0 2± 2 3)μm,细胞长度下降的百分数为 8.7% ,1 5.7%和 1 6.6% ,与对照组相比有显著差异 (P<0 .0 5) ;在剂量为 0 .2 2～ 0 .89g·L-1 范围内 ,番泻叶对平滑肌细胞的收缩作用逐渐增强 (P<0 .0 5) ,呈剂量 -效应关系 .结论　番泻叶提取物对豚鼠结肠平滑肌细胞有直接的收缩作用

Contraction effect of senna extract on colon smooth muscle cells in guinea pig

AIM To investigate the effect of senna extract on contraction of colonic smooth muscle cells of guinea pig. METHODS Smooth muscle cells were isolated from the colon of the guinea pig. Contraction in response to the senna extract, measured by micrometry, was observed. RESULTS Isolated smooth muscle cells were obtained. When incubated with 0.22, 0.44, 0.89 g*L-1 of senna extract, the mean cell length was (93±20), (86±25), (85±15) μm, while that of the control was (102±23) μm, and the percentage of shortage was 8.7%, 15.7% and 16.6% respectively. The treated cell length was significantly shorter (P<0.05). Cell contraction increased in accordance with the concentration of the senna extract in a dose dependent manner. CONCLUSION Senna extract has a direct contractile effect on the colonic smooth muscle cells of guinea pig.