CCL1/CCR8在哮喘小鼠肺组织中的表达及地塞米松对其作用的研究

目的观察哮喘小鼠肺组织CCL1/CCR8 mRNA的表达及糖皮质激素对其表达的影响,探讨CCL1/CCR8在哮喘发病机制中的作用。方法30只小鼠随机分为对照组、哮喘组和地塞米松组,每组10只。哮喘组和地塞米松组用卵白蛋白致敏激发建立哮喘模型,地塞米松组给予腹腔注射地塞米松(2mg/kg),对照组均以生理盐水替代。测定BALF中细胞总数和分类计数,采用酶联免疫法检测BALF中IL-4水平,RT-PCR检测肺组织CCR8 mRNA和CCL1 mRNA的表达。结果对照组、哮喘组和地塞米松组BALF中嗜酸粒细胞百分比(0.6±0.1)%、(32.4±3.4)%和(8.9±0.9)%,P0.01]、淋巴细胞百分比(2.9±0.5)%、(14.0±3.0)%和(6.3±0.6)%,P0.01]、IL-4浓度(5.16±1.23)pg/mL、(47.67±11.67)pg/mL和(19.76±4.72)pg/mL,P0.01]差异均有统计学意义,其中哮喘组较对照组显著增高(P0.01),地塞米松组较哮喘组显著降低但仍显著高于对照组(P0.01)。对照组、哮喘组和地塞米松组CCL1mRNA表达(0.11±0.06、0.76±0.16和0.53±0.13,P0.01)、CCR8 mRNA表达(0.18±0.09、1.26±0.13和0.79±0.12,P0.01)差异均有统计学意义,其中哮喘组较对照组显著增高(P0.01),地塞米松组较哮喘组显著降低但仍显著高于对照组(P0.01)。结论CCL1/CCR8在哮喘小鼠肺组织中的基因表达增强。糖皮质激素可能通过减少CCL1/CCR8的基因表达,减轻哮喘气道炎症。

Effects of Dexamethasone on the Expression of CCL1 and CCR8 in Asthmatic Mice

Objectives To observe the expression of CCL1/CCR8 mRNA in murine lung tissue of bronchial asthma and effects of glucocorticoids on their expression. Methods Thirthy healthy mice were randomly divided into a control group, an asthma group, and a dexamethasone group, with 10 mice in each group. The sensitized murine asthma model was induced by ovalbumin sensitization and challenge, and the dexamethasone group were peritoneally injected with dexamethasone （2 mg/kg）. Total and differential cell counts in BALF were measured. IL-4 Level in BALF was evaluated by ELSA. The expression of CCL1 and CCR8 mRNA in the lungs were measured by semi-quantitative RT-PCR. Results The percentage of eosinophils, lymphocyte and IL-4 level in the asthma group increased significantly compared with the control group,and which in the dexamethasone group decreased significantly compared with the asthma group arid still higher than the control group（ all P 〈 0. 01 ）. The expression of CCL1 and CCR8 mRNA had the same tendency （all P 〈 0. 01 ）. Conclusions The gene expression of CCL1/CCR8 is up-regulated in allergic asthma mice. Glucocorticoids can relieve airway inflammation of asthma probably by inhibiting CCL1/CCR8 expression.