一氧化氮/内皮素平衡关系与移植肝功能的相关研究

目的　研究大鼠肝移植术后早期一氧化氮 (NO ) /内皮素 (ET )平衡关系的改变 ,探讨其与肝功能的相关性 ,观察前列腺素E1 (PGE1 )的干预作用。方法　大鼠随机分为 4组 (各组n =8) :(1)正常对照组 (A组 ) ;(2 )手术对照组 (B组 ) ;(3 )实验组 (C组 ) ;(4 )PGE1 干预组 (D组 )。采用“双袖套法”建立肝移植模型。检测和比较受体术后 6h血液ALT ,AST ,NO和ET水平 ,观察肝脏组织学改变。结果　术后 6hB ,D组全部存活 (10 0 % ) ,C组存活 5只 (62 .5 % )。与C组比较 ,D组ALT ,AST ,ET明显降低 (P <0 .0 5 ) ,NO明显升高 (P <0 .0 5 )。NO ,ET及NO /ET值与ALT呈现明显的相关性 (依次为r =-0 .681,0 .793和 -0 .73 2 )。C组肝脏见明显的组织学损害 ,B ,D组组织结构基本保存完好。结论　肝移植术后早期血液NO ,ET水平及NO /ET比例关系 ,较好地反映了肝脏内皮细胞的功能状况。PGE1 可减轻肝血窦内皮细胞的损害 ,保护了移植肝功能。

Correlation of the ratio of nitric oxide/endothelin and liver graft function

Objective To study the alternation of nitric oxide(NO)/endothelin(ET) ratio in early stage of liver transplantation in rats, to investigate its correlation with liver function, and to observe the effect of prostaglandin E 1(PGE 1) on the graft function. Methods Wister rats randomly divided into 4 group ( n =8 in each group):(1)Normal control group(group A);(2)Operative control group (group B);(3)Experimental group(group C);(4)PEG 1 treatment group (group D).Orthotopic liver transplantation(OLT) model was set up by "two-cuff" method.ALT, AST, NO and ET level in blood and histological change of liver graft were detected and compared at 6th hour postoperatively. Results All the rats survived 6h in group B and D(100%),but only 5 survived in group C ( 62.5 %). Comparing with group C, ALT, AST and ET decreased and NO increased significantly ( P <0.05) in group D. The levels of NO and ET, and NO/ET ratio were correlated with ALT closely ( r =-0.681, 0.793 and -0.732 respectively). Obviously histological injury was observed in group C, while morphological appearance was better preserved in group B and D. Conclusions Concentration of NO, ET and NO/ET ratio in recipient blood more conrrectly reflect the function of sinusoid endothelial cell of the graft. PGE 1 can relieves endothelial injury and maintains the function of liver graft.