| 反相高效液相色谱法测定甘草酸脂质体中甘草酸的含量 |
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| 引用本文： | 扈本荃,程建峰,刘梅,贺建荣,王弼时,杨胜. 反相高效液相色谱法测定甘草酸脂质体中甘草酸的含量[J]. 中国药房, 2006, 17(9): 692-693 |
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| 作者姓名： | 扈本荃 程建峰 刘梅 贺建荣 王弼时 杨胜 |
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| 作者单位： | 第四军医大学唐都医院药学部,西安市,710038 |
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| 基金项目： | 陕西省中医药管理局招标项目 |
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| 摘 要： | 目的:建立以反相高效液相色谱法测定甘草酸脂质体中甘草酸含量的方法。方法:色谱柱为Shim-PackVP-ODS,流动相为甲醇-磷酸盐缓冲液(pH=2.6,68∶32),检测波长为254nm,流速为1ml/min。结果:甘草酸与辅料及溶剂峰分离良好;甘草酸检测浓度在1～100μg/ml范围内线性关系良好(r=0.9999,n=5);平均回收率为97.56%(RSD=1.03%)。结论:本法准确、灵敏,可用于甘草酸脂质体中甘草酸的含量测定。
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| 关 键 词： | 甘草酸 脂质体 反相高效液相色谱法 含量 包封率 |
| 文章编号： | 1001-0408(2006)09-0692-02 |
| 收稿时间： | 2005-06-08 |
| 修稿时间： | 2005-09-22 |
Content Determination of Glycyrrhizic Acid in Glycyrrhizin Liposome by RP- HPLC |
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| HU Benquan,CHENG Jianfeng,LIU Mei,HE Jianrong,WANG Bishi,YANG Sheng. Content Determination of Glycyrrhizic Acid in Glycyrrhizin Liposome by RP- HPLC[J]. China Pharmacy, 2006, 17(9): 692-693 |
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| Authors: | HU Benquan CHENG Jianfeng LIU Mei HE Jianrong WANG Bishi YANG Sheng |
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| Abstract: | OBJECTIVE:To establish an RP-HPLC method for the content determination of glycyrrhizic acid in gly-cyrrhizin liposome.METHODS:The separation was performed on Shim-pack VP-ODS column with methanol-buffer phosphate(pH=2.6,68∶32)as the mobile phase,the detection wavelength was254nm and the flow rate was1ml/min.RE-SULTS:Glycyrrhizic acid was well-separated with peaks of the adjuvants and the solvent.The linear range for glycyrrhizic acid was1～100μg/ml(r=0.9999,n=5).The average recovery was97.56%(RSD=1.03%).CONCLUSION:This method is accurate and sensitive,and suitable for the content determination of glycyrrhizic acid in glycyrrhizin liposome. |
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| Keywords: | Glycyrrhizie acid Liposome RP - HP LC Contents Entrapment rate |
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