| TRIM22蛋白与A型流感病毒复制的相互关系研究 |
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| 引用本文： | 李卫中,周健祥,曾祥兴,陈幼莹,李康生. TRIM22蛋白与A型流感病毒复制的相互关系研究[J]. 汕头大学医学院学报, 2010, 23(4): 198-201 |
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| 作者姓名： | 李卫中 周健祥 曾祥兴 陈幼莹 李康生 |
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| 作者单位： | 汕头大学医学院微生物学与免疫学教研室; |
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| 基金项目： | 国家自然科学基金资助项目，广东省基金资助项目 |
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| 摘 要： | 目的：研究TRIM22蛋白对A型流感病毒复制能力的影响。方法：RT-PCR扩增人TRIM22全长编码序列并制备TRIM22真核表达质粒;通过绘制病毒生长曲线及直接做空斑的方法检测外源性表达TRIM22情况下,两种不同流感病毒株PR8（H1N1）和A/ST/364/2005（H3N2）在MDCK细胞中的复制能力。结果：成功构建TRIM22真核表达质粒;两种不同亚型流感病毒在过表达TRIM22的情况下,其在MDCK细胞中的生长曲线及空斑大小与阴性对照组相似。结论：TRIM22不能有效抑制不同亚型A型流感病毒的复制。
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| 关 键 词： | TRIM22蛋白 流感病毒 复制 |
Study on the Relationship between TRIM22 Protein and Replication of Influenza A Viruses |
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| LI Wei-zhong,ZHOU Jian-xiang,ZENG Xiang-xing,CHEN You-ying,LI Kang-sheng. Study on the Relationship between TRIM22 Protein and Replication of Influenza A Viruses[J]. Journal of Shantou University Medical College, 2010, 23(4): 198-201 |
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| Authors: | LI Wei-zhong ZHOU Jian-xiang ZENG Xiang-xing CHEN You-ying LI Kang-sheng |
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| Affiliation: | LI Wei-zhong,ZHOU Jian-xiang,ZENG Xiang-xing,CHEN You-ying,LI Kang-sheng(Department of Microbiology and Immunology,Shantou University Medical College,Shantou 515041,China) |
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| Abstract: | Objective:To investigate the effect of TRIM22 protein on the replication of influenza A viruses.Methods:Full-length encoding sequence of human TRIM22 was cloned by RT-PCR and a recombinant plasmid expressing TRIM22 was generated.Two different influenza A viruses(PR8(H1N1)and A/ST/364/2005(H3N2))were used for the subsequent analysis of viral growth curves and plaque sizes in MDCK cells upon the exogenous expression of TRIM22 or not.Results:A eukaryotic plasmid expressing human TRIM22 protein was successfully... |
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| Keywords: | TRIM22 protein influenza virus replication |
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