| Abstract: | We studied the regulation of the expression of complement regulatory proteins, membrane cofactor protein (MCP), decay accelerating factor (DAF) and CD59, on human keratinocytes by supernatant of activated mononuclear cells and by some individual cytokines present therein. Cultured keratinocytes expressed MCP, DAF and CD59. Supernatant of activated mononuclear cells and recombinant forms of transforming growth factor (TGF)-β variants (β1, β2 and β3) up-regulated MCP and CD59 but not DAF. Recombinant IL-1α, IL-2, IL-6, TNF-α and IFN-γ had no influence. TGF-β present in the supernatant was likely responsible for up-regulation of MCP and CD59. A monoclonal anti-TGF-β antibody, which neutralized TGF-β1, -β2 and -β3, did not inhibit the up-regulation of MCP and CD59 by the supernatant. These results indicated that TGF-β and an additional factor(s) present in the supernatant may be responsible for up-regulating the expression of MCP and CD59 on keratinocytes; both may be acting non-synergistically. |
