| 大鼠脊髓损伤后神经生长抑制因子Nogo-A的表达 |
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| 引用本文： | 王翠芳,毋崇岭,马延超,陈文华. 大鼠脊髓损伤后神经生长抑制因子Nogo-A的表达[J]. 临床骨科杂志, 2008, 11(6): 572-575 |
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| 作者姓名： | 王翠芳 毋崇岭 马延超 陈文华 |
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| 作者单位： | 兰州大学第二附属医院骨科研究所,甘肃,兰州,730030;兰州大学第二附属医院骨科研究所,甘肃,兰州,730030;兰州大学第二附属医院骨科研究所,甘肃,兰州,730030;兰州大学第二附属医院骨科研究所,甘肃,兰州,730030 |
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| 摘 要： | 目的研究神经生长抑制因子Nogo—A在大鼠脊髓损伤组织中不同时间点的表达情况．探讨Nogo—A基因在脊髓损伤时对神经再生的作用．方法建立急性脊髓损伤模型，采用原位杂交技术结合免疫组化技术检测。结果原位杂交法显示：Nogo-A mRNA在脊髓损伤后1d表达下降，3d升高，7d表达最高.免疫组化显示：Nogo—A含量在脊髓损伤后1d表达下降，3d开始上升，7d表达最高.结论Nogo-A在脊髓损伤后1d表达不明显，而后随时间的延长表达逐渐增加．表明脊髓损伤后Nogo-A表达先降低后升高．可能是造成成年哺乳动物损伤后神经再生障碍的重要原因之一.
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| 关 键 词： | 神经再生 脊髓损伤 Nogo-A 原位杂交 免疫组化技术 |
Expression of nerve growth inhibiting factor Nogo-A after spinal cord injury in rats |
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| WANG Cui-fang,WU Chong-ling,MA Yan-chao,CHEN Wen-hua. Expression of nerve growth inhibiting factor Nogo-A after spinal cord injury in rats[J]. Journal of Clinical Orthopaedics, 2008, 11(6): 572-575 |
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| Authors: | WANG Cui-fang WU Chong-ling MA Yan-chao CHEN Wen-hua |
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| Affiliation: | Institute of Orthopaedics;the 2nd Affiliated Hospital of Lanzhou University;Lanzhou;Gansu 730030;China |
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| Abstract: | Objective To investigate the dynamic changes of Nogo-A expression after spinal cord injury in rats.Methods The model of spinal cord injury was established and expression of Nogo-A mRNA was measured by immunohistochemistry and in situ hybridization.Results Nogo-A mRNA expression was decreased at the 1th day and increased significantly at the 7th day,and changed little at the 3th day.Nogo-A protein expression was decreased at the 1th day and increased significantly at the 3th and 7th day.Conclusions Expressio... |
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| Keywords: | Nogo-A |
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