Detection of antibodies to 65 KD heat shock protein and to human superoxide dismutase in autoimmune hepatitis-molecular mimicry between 65 KD heat shock protein and superoxide dismutase |
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Authors: | M. Miyata A. Kogure H. Sato E. Kodama H. Watanabe H. Ohira M. Kuroda T. Takagi Y. Sato R. Kasukawa |
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Affiliation: | (1) Department of Internal Medicine II, Fukushima Medical College, 1 Hikarigaoka, 96012 Fukushima, JAPAN |
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Abstract: | Summary The antibody to 65 KD mycobacterial heat shock protein (HSP65) and antibody to human superoxide dismutase (H-SOD) were measured by ELISA in patients with autoimmune hepatitis (AIH), and results were compared with those of patients with chronic active hepatitis C (CAH-C) or systemic lupus erythematosus (SLE) and normal subjects (NS). Patients with AIH had significantly higher OD values of anti-HSP65 antibody and anti-H-SOD antibody compared with those of patients with CAH-C or SLE and NS. OD values of anti-HSP65 antibody were correlated with those of anti-SOD antibody. Affinity-purified anti-SOD antibody reacted with HSP65. Analysis of the amino acid sequence of human SOD showed that 7 segments, corresponding to r to 25 amino acid residues, exhibited 50 to 71% homology with that of mycobacterial HSP65. |
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Keywords: | Autoimmune Hepatitis Mycobacterial 65 KD Heat Shock Protein Superoxide Dismutase Human 60 KD Heat Shock Protein Molecular Mimicry |
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