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The influence of CaMKII and ERK phosphorylation on BDNF changes observed in mice selectively devoid of CREB in serotonergic or noradrenergic neurons
Authors:Katarzyna Rafa-Zabłocka  Grzegorz Kreiner  Monika Bagińska  Irena Nalepa
Affiliation:Department of Brain Biochemistry, Maj Institute of Pharmacology, Polish Academy of Sciences, Kraków, Poland
Abstract:
Keywords:Corresponding author.  BDNF  brain derived neurotrophic factor  BCA  bicinchoninic acid  BSA  bovine serum albumin  CaMKII  Ca2+/calmodulin-dependent protein kinase II  CREB  cyclic AMP response element binding?protein  CREM  cyclic AMP response element modulator  DBH  dopamine β-hydroxylase  DMI  desipramine  ERK  extracellular signal-regulated kinase  ER  estrogen receptor  ERT2  mutant estrogen ligand-binding domain  FLX  fluoxetine  GAPDH  glyceraldehyde 3-phosphate dehydrogenase  HIP  hippocampus  KO  knock out  LC  locus coeruleus  MAP  mitogen-activated protein kinase  PFC  prefrontal cortex  RIPA  radioimmunoprecipitation assay buffer  RN  raphe nuclei  SAL  saline  SSRI  selective serotonin reuptake inhibitor  TPH  tryptophan hydroxylase-2  TrkB  tropomyosin receptor kinase B  TST  tail suspension test  BDNF  CREB  Desipramine  Fluoxetine  Noradrenergic system  Serotonergic system
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