铁缺乏对红细胞膜带3蛋白结构相关酶的影响

方法：测定了１０种红细胞酶。结果：１．缺铁性贫血大鼠红细胞中大多数胞浆酶活力呈代偿性增高，而带３膜绑酶无显著增高表现。２．酶的稳定性在胞浆酶和膜绑酶均明显下降，稳定性测值可作为反映红细胞寿命的一项指标。３．铁缺乏使与ＣＤＢ３结合的ＰＦＫ、Ｇ３ＰＤ的活力与稳定性显著下降；带３蛋白分子降解、含量下降可能是缺铁红细胞膜绑酶病变的直接原因。４．适当的铁剂治疗对于缺铁红细胞酶稳定性的恢复有肯定作用。

TX EFFECTS OF IRON DEFICIENCY ON THE ENZYMES BINDING WITH BAND 3 PROTEIN IN ERYTHROCYTE MEMBRANE

The　cytoplasmic　domain　of　band　3　protein,the　anion　transport　protein　of　the　red　cell,is　the　high　affinity　binding　site　on　the　red　cell　membrane　for　glycolytic　enzymes,such　as　glyceraldehyde 3 phosphate　dehydrogenase,phosphofructokinase　and　aldolase.The　membrane　capacity　for　the　three　enzymes　is　coincident　with　the　band　3　protein　content.Previous　studies　have　shown　that　the　content　of　band　3　protein　was　markedly　reduced　and　the　anion　transport　function　of　band　3　was　strikingly　weakned　in　the　red　cell　of　rat　with　iron　deficient　anemia.The　effects　of　iron　deficiency　on　the　enzymes　binding　with　band　3　protein　in　membrane　and　enzymes　in　cytoplasma　of　erythrocyte　were　found　that　a　compensative　increase　in　the　activity　of　the　most　inspected　enzymes　in　cytoplasma　(totally　10)　of　IDA　red　cells,which,however,　was　not　seen　in　the　enzymes　binding　with　band　3　in　membrane.The　stability　of　enzymes　either　in　cytoplasma　or　binding　with　membrane　was　markedly　decreased　in　IDA　red　cell　especially　glyceraldehyde 3 phosphate　dehydrogenase,phosphofructokinase,pyrimidine　5 nucleoside,glucose 6 phosphate　dehydrogenase　and　pyruvate　kinase,which　indicated　that　the　stabi lity　of　enzyme　in　red　cell　might　be　associated　with　the　life　span　of　red　cell.The　reduction　in　both　activity　and　stability　of　glyceraldehyde 3 phosphate　dehydrogenase　and　phosphofructokinase　binding　with　band　3　might　result　from　the　reduction　of　band　3　content　due　to　the　cleavage　of　the　protein　into　band　3 64kD　in　IDA　red　cells.It　is　suggested　that　the　stability　of　enzymes　in　red　cell　might　return　to　normal　by　proper　iron　therapy.