支链氨基酸对大鼠心肌缺血损伤的防护作用

目的：观察支链氨基酸（ＢＣＡＡ）对大鼠心肌缺血损伤的预防作用。方法：选用Ｗｉｓｔａｒ大鼠３０只，随机分成三个组，每组１０只。Ａ组：正常对照组；Ｂ组：异丙肾上腺素（ＩＳＯ）对照组；Ｃ组：ＩＳＯ＋ＢＣＡＡ组，其中Ｂ、Ｃ组用ＩＳＯ造成大鼠心肌缺血性损伤。用酶试剂盒和原子吸收法测定了血清、心肌中ＬＤＨ、ＧＯＴ、α－ＨＢＤＨ、ＣＫ、ＰＫ的酶活力及心肌中Ｋ＋、Ｍｇ２＋、Ｃａ２＋离子的含量。结果：Ｂ组大鼠血清和心肌中ＬＤＨ、ＧＯＴ、α－ＨＢＤＨ、ＣＫ、ＰＫ的酶活力均明显升高，心肌中Ｃａ２＋的含量也显著升高，而Ｍｇ２＋的含量明显降低，Ｋ＋含量也有下降的趋势，但变化不显著。Ｃ组大鼠血清中ＬＤＨ、α－ＨＢＤＨ、ＣＫ、ＰＫ的酶活力明显低于Ｂ组，而且ＣＫ的活力接近正常水平；心肌中ＬＤＨ、ＧＯＴ、α－ＨＢＤＨ、ＣＫ的活力均低于Ｂ组并达到正常水平。补充ＢＣＡＡ后能有效地拮抗心肌细胞内钙的增加，使心肌内Ｃａ２＋含量明显低于Ｂ组，与正常对照组无显著差异，但ＢＣＡＡ不能升高受损心肌中Ｍｇ２＋的含量。结论：给予ＢＣＡＡ对心脏缺血性损伤具有一定的防护效果

PROTECTION OF BRANCHED CHAIN AMINO ACIDS ON ISCHEMIC MYOCARDIAL INJURY IN RAT

In order to study the preventive effects of BCAA on ischemic myocardium in rat,30 Wistar rats were randomly divided into 3 groups and 10 rats in each group.Group A:control group;group B:ISO group;group C:ISO+BCAA group.Isoproternol (ISO) induced acute ischemic myocardial injury in group B and group C.The activity of LDH,GOT,α HBDH,CK,PK in serum and myocardium and the contents of K +,Mg 2+ ,Ca 2+ in myocardium were measured by using enzymic reagent boxes and atomic absorption spectrum respectively.The results showed that the activity of LDH,GOT,α-HBDH,CK,PK in serum and in myocardium was significantly increased in group B.The content of Ca 2+ in myocardium was also significantly increased in group B while the content of Mg 2+ in myocardium was significantly decreased.The content of K + in myocardium was insignificantly decreased.In group C,the activity of LDH,α HBDH,PK,CK in serum was significantly lower than in group B and the activity of LDH,GOT,α HBDH,PK,CK in myocardium was also significantly lower than in group B which showed no significant difference with the normal group.Supplementation of BCAA could effectively antagonize the increase of calcium in myocardium,but could not increase the level of Mg 2+ .It was concluded that supplementation of BCAA could produce some protective effects on ischemic myocardium.