不同维生素E水平对四氯化碳肝损害的影响

方法：大鼠经２９天喂养造成不同ＶＥ水平的模型。一次腹腔注射０．６５ｍｌ／ｋｇ四氯化碳（ＣＣｌ４）。结果：２４小时后，肝匀浆中丙二醛（ＭＤＡ）水平明显增高，ＶＥ缺乏组升高更明显，ＶＥ添加组ＭＤＡ明显下降。肝匀浆中总巯基、非蛋白巯基、蛋白巯基在染毒后未发生明显改变，也未发现不同水平ＶＥ对其有影响。ＣＣｌ４染毒后，血清丙氨酸转氨酶（ＡＬＴ）明显升高：随着饲料ＶＥ水平增高，ＡＬＴ上升幅度增大，病理检查变性坏死面积稍有增加，提示ＶＥ似有促进肝细胞坏死作用，脂质过氧化可能不是ＣＣｌ４致肝细胞坏死的唯一机理。肝匀浆甘油三酯（ＴＧ）在染毒后明显升高，说明ＴＧ在肝脏中蓄积。饲料ＶＥ增高，其蓄积程度减轻，但对血清中ＴＧ影响不大。ＶＥ也可降低血清中胆固醇的含量。结论：ＶＥ有预防ＣＣｌ４所致ＴＧ在肝中蓄积和脂肪肝形成的作用。

THE EFFECT OF DIFFERENT VITAMIN E INTAKE LEVELS ON LIVER DAMAGE OF RATS INDUCED BY CARBON TETRACHLORIDE

Thirty two weanling SD rats were divided into 4 groups and were given diets containg different levels of Vitamin E for 29 days.The experimental rats were injected CCl 4 (0.65 ml/kg) at the 30 day.24 hours after CCl 4 there were significantly increases of MDA in the rat liver and the amount of MDA was higher in the Vitamin E deficiency group compared to the vitamin E supplemented group.There were no changes of T SH,NP SH,P SH in liver of experimental rats.Serum ALT were significantly increased after CCl 4 administration and the more vitamin E level of diets,the more serum ALT level.Histopathological examination also supported this change.TG in liver was increased after CCl 4 administration.But when the vitamin E level was higher,the amount of ALT was lower.Our experiment suggesed that lipid peroxidation was not the only machanism of liver damage induced by CCl 4 and vitamin E should prevent TG deposits in liver.