硒和维生素E对人白血病周期的影响

方法：应用体外细胞培养法，流式细胞术（ＦＣＭ）研究硒和ＶＥ对人髓系白血病细胞株ＨＬ－６０和非髓系白血病细胞株Ｋ５６２细胞增殖和细胞周期分布的影响。结果：Ｓｅ（５～１１μｍｏｌ／Ｌ）对于ＨＬ－６０，Ｋ５６２细胞的增殖均有显著性抑制作用，ＦＣＭ表明，在Ｓｅ（８μｍｏｌ／Ｌ）作用６～７２ｈ后，ＨＬ－６０和Ｋ５６２细胞分别出现Ｇ１和Ｓ期阻滞现象。ＶＥ在１００～３００μｍｏｌ／Ｌ范围内对ＨＬ－６０细胞具有显著性抑制作用，而在１０～３００μｍｏｌ／Ｌ范围内对Ｋ５６２细胞同样具有显著性抑制作用，且呈剂量相关效应。ＶＥ（１００μｍｏｌ／Ｌ）作用６～７２ｈ后，ＨＬ－６０，Ｋ５６２细胞分别被阻滞于Ｓ期和Ｇ１期。值得注意的是Ｓｅ与ＶＥ对ＨＬ－６０和Ｋ５６２细胞周期分布的影响恰恰相反。结论：硒的抗癌作用和ＶＥ的抗癌作用是互补的，不能互相替代

EFFECTS OF SELENIUM AND VITAMIN E ON PROLIFERATION AND CELL CYCLE OF LEUKEMIC CELL LINES HL 60 AND K562

Myelogenous leukemia HL 60 and non myelogenous leukemia K562 cells were incubated with different concentrations of selenium(Se) and vitamin E(VE). To both HL 60 and K562, Se at concentrations from 5 to 11 μmol/L showed inhibitory effect on proliferation. From the results of flowcytometric method(FCM), a 6 to 72 hours exposure of HL 60 and K562 cells to Se(8 μmol/L) caused cell cycle arrested at G 1 and S phase respectively. Similarly, VE in concentrations from 100 to 300 μmol/L inhibited cell growth of HL 60 significantly; furthermore, in concentrations from 10 to 300 μmol/L, VE showed inhibitory effect on K562 with dose dependence. The results of FCM revealed that HL 60 and K562 cells were arrested at S and G 1 phase respectively, as exposed to VE(100 μmol/L) for 6 to 72 hours. It is interesting to note that the arrested phase of VE on the two cell lines were entirely in contrast to Se, though VE and Se were known to have synergistic anti cancer action. These observations suggested that the anti cacer effects of Se and VE were not replaceable each other but rather complementary.