反相液相色谱法同时测定食物中维生素K_1和K_2

方法：食物中ＶＫ１、ＶＫ２用己烷提取，以石油醚乙醚（８５１５）为展开剂，于ＧＦ２５４ｎｍ硅胶薄板上纯化，正己烷萃取，离心，８０℃水浴蒸干。用甲醇溶解残留物，经ＯＤＳ－Ｃ１８反相柱，以甲醇为流动相。结果：在１．５ｍｌ／ｍｉｎ的流速下，选择２５４ｎｍ检测，ＶＫ１、ＶＫ２能很好地分离，ＶＫ１和ＶＫ２两者的回收率分别为８８．３％～９０．８％和８９．８％～９１．５％，变异系数为４．８２％～５．４９％。结论：反相液相色谱法简单、准确、适合于食物中ＶＫ１、ＶＫ２的分析。

TX THE SIMULTANEOUS DETERMINATION OF VITAMIN K1 AND VITAMIN K2 IN FOOD BY RPLC

A　reversed　phase　high　performance　liquid　chromatographic　method　had　been　developed　for　the　simultaneous　determination　of　vitamin　K1　and　vitanim　K2　in　foods.　K　vitamins　of　food　were　extracted　with　n haxane.　The　extracted　vitamins　were　purified　with　a　thin layer　chromatography　and　then　analysed　on　a　column　of　ODS　C18,　methyl　alchol　was　used　as　mobile　phase.　K　vitamins　were　detected　at　254nm　by　UV　spectrophotometer.　The　recoveries　of　VK1　and　VK2　were　88.3%-90.8%　and　89.9%-91.5%　respectively,　the　coefficients　of　variation　were　4.82%　to　5.49%.　This　method　was　simple,　rapid　and　accurate.