新生儿不动杆菌败血症的药敏试验与质粒关系

目的 了解新生儿不动杆菌败血症暴发流行的耐药性和质粒的关系。方法 收集来自病儿血培养104株不动杆菌，对16种抗菌药物进行药敏试验和质粒检测。结果 104株均检出质粒，56株流行菌株含有4个相同的质粒（30．1、3．1、2．5、2kb）．非流行株的质粒数目不等（1—6个）。细菌的耐药性与质粒密切相关，对庆大霉素、卡那霉素、妥布霉素、蒜啶酸、头孢孟多、复方新诺明耐药率流行菌株显著高于非流行菌株，在多重耐药菌株构成比中流行菌株也显著高于非流行菌株。结论新生儿病房流行的不动杆菌呈多重耐药性，耐药率流行型菌株高于非流行型菌株；不动杆菌感染选择头孢噻肟和阿米卡星为宜。临床流行病学调查中质粒分析结合药物敏感试验效果较好。

The plasmid analysis and antibiotic susceptibility testing of acintobacter calcoaceticus in neonatal word

Objective An outbreak of multiresistant acinetobacter calcoaceticus septicaemia occured in the neonatal unit of Hospital,to investigate the relation between the plasmid and the resistance.Methods The plasmid analysis and drug sensitivity tests were used to conduct an epidemiological study of 104 A.calcoaceticus and to establish their relationship.Results 6 epidemic strains were A.anitratus which contained the same plasmid profiles(30.1,3.1,2.5 and 2 Kb),Antibacterial drug resistance had close relation to the plasmid,The epidemic strains were higher rate of resistance drug than no epidemic strains.Conclusion Amikacin and cefotaxime were also quite useful in treatment A.calcoaceticus infection.The combined use of antimicrobial drug susceptibility and plasinid analysis could be satisfactory for clinical epidemiological investigation.