Characterization of multiphoton microscopy in the bone marrow following intravital laser osteotomy |
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| Authors: | Rapha?l Turcotte Clemens Alt Luke J. Mortensen Charles P. Lin |
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| Affiliation: | 1.Advanced Microscopy Program, Center for Systems Biology and Wellman Center for Photomedicine, Massachusetts General Hospital, Harvard Medical School, CPZN 8238, 185 Cambridge Street, Boston, MA 02114, USA;2.Department of Biomedical Engineering, Boston University, Boston, MA 02215, USA |
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| Abstract: | The bone marrow is an important site where all blood cells are formed from hematopoietic stem cells and where hematologic malignancies such as leukemia emerge. It is also a frequent site for metastasis of solid tumors such as breast cancer and prostate cancer. Intravital microscopy is a powerful tool for studying the bone marrow with single cell and sub-cellular resolution. To improve optical access to this rich biological environment, plasma-mediated laser ablation with sub-microjoule femtosecond pulses was used to thin cortical bone. By locally removing a superficial layer of bone (local laser osteotomy), significant improvements in multiphoton imaging were observed in individual bone marrow compartments in vivo. This work demonstrates the utility of scanning laser ablation of hard tissue with sub-microjoule pulses as a preparatory step to imaging.OCIS codes: (120.0120) Instrumentation, measurement, and metrology; (170.1020) Ablation of tissue; (180.5810) Scanning microscopy |
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