前列地尔防治大鼠马兜铃酸肾病纤维化

目的:观察前列地尔对马兜铃酸肾病(AAN)纤维化的防治作用。方法:Wistar大鼠25只,随机分3组,对照组(n=5)予灌服饮用水10mL·kg-1·d-1,模型组及治疗组(均n=10)均予关木通水煎剂20g·kg-1·d-1,共计12wk。治疗组8wk后同时予尾静脉注射前列地尔4μg·kg-1·d-1,共2wk。12wk时观察各组大鼠尿素氮(BUN)和肌酐(Cr)、肾组织转化生长因子β1(TGF-β1)、结缔组织生长因子(CTGF)和纤连蛋白(FN)的变化。结果:模型组大鼠BUN,Cr较对照组和治疗组明显升高(P<0.01);模型组中TGF-β1,CTGF和FN光密度明显高于对照组和治疗组(P<0.05);治疗组大鼠肾脏病理显示肾损害较模型组明显减轻。结论:前列地尔能使AAN大鼠肾组织TGF-β1,CTGF和FN表达下降,减轻马兜铃酸肾病纤维化程度。

Alprostadil preventing fibrosis of aristolochic acid nephropathy in rats

AIM: To evaluate the mechanism of the effect of alprostadil on preve nting the fibrosis of aristolochic acid nephropathy (AAN). METHODS: Twenty-fi ve Wistar rats were divided randomly into 3 groups. The rats in the control group (n=5) were fed with water (10 mL·kg -1 ·d -1 ). The rats in the m od el group (n=10) and the therapy group (n=10) were fed with Aristo lochia manshuriensis( 20 g· kg -1 · d -1 ) for 12 wk respectively and furthermore the rats in the therapy group were simu ltaneously given alprostadil (4 μg·kg -1 ·d -1 ) at the 8th week the se afterward via the caudal vein. The serum levels of BUN, Cr and TGF- β_1, CTGF, F N in nephridial tissue were analyzed after 12 wk respectively together with rena l histology analysis. RESULTS: The hemoglobin and weight in model group was significantly lower and urinary protein excretion was higher than that of control group. BUN and Cr in m odel group were significantly higher than those in the other two groups (P< 0.01 ). Renal h istologic damage in therapy group was obviously less serious than that of the mo del group. The optical densities of TGF-β_1, CTGF and FN with immunohistochem ica l in model group changes were significantly higher than those of the other two g roups (P< 0.05 ). CONCLUSION: The results indicate that alprostadil can prevent a nd cure the fibrosis of AAN by lowing the expression o f TGF-β_1, CTGF and FN in renal tissue of AAN rat.