吉西他滨对人胆管癌裸鼠移植瘤模型放射增敏作用的实验研究

目的评价吉西他滨对人胆管癌裸鼠移植瘤的放射增敏作用。方法建立人胆管癌裸鼠移植瘤模型,60只荷瘤鼠随机分为4组:空白对照组、单纯药物组、单纯放射治疗组、吉西他滨联合放射治疗组。单纯照射组和联合治疗组根据照射剂量不同各分为3个亚组。对照组经腹腔注射生理盐水,单纯药物组经腹腔注射吉西他滨50mg/kg;联合治疗组经腹腔注射吉西他滨50mg/kg,24h后分别进行单次10、15和20Gy的照射;隔日测量移植瘤的最大径及最小径,计算肿瘤体积及生长延缓天数等,利用增敏系数(EF)评价吉西他滨的放射增敏作用。结果单纯照射组各亚组肿瘤生长受到不同程度的抑制;联合治疗组较其他实验组肿瘤生长明显受到抑制,10、15和20Gy3个亚组的EF值分别为1.14、1.74和2.08;20Gy亚组延缓天数最长,且大于单纯药物组和单纯照射组延缓天数之和。结论照射组前24h给予吉西他滨对人胆管癌裸鼠移植瘤具有明显的放射增敏效应。

Radioenhancement on cholangiocarcinoma by gemcitabine in vivo

Objective To evaluate the radioenhancement of gemcitabine on human cholangiocarcinoma grafted in nuke mice. Methods Tumour-bearing model was established by injecting cholangiocarcinoma cells (QBC939)to nude mice. Mice bearing chalongiocarcinoma were randomly divided into four groups: control group,gemcitabine group,radiation group,combination of gemcitabine and radiation group. The radiation group and combination group were divided into 3 subgroups according to the dose of radiation(10 Gy,15 Gy,20 Gy). Each group comprised 15 mice. Gemcitabine was injected as 50 mg/kg. Tumors were measured every other day using vernier calipers. The tumor volume and growth delay were calculated. The enhancement factor(EF)was used to evaluate the radiation enhancement. Results Tumor growth inhibition in combination group was higher than those of radiation group and gemcitabin group(P < 0. 05). The values of EF were 1.22,1.39,1.74 in subgroups(10 Gy,15 Gy,20 Gy)of GR group,respectively. The longest time of tumor growth delay were 54 days in combination group. Conclusions Gemcitabine possesses radioenhancing property for cholangiocarcinoma. The highest EF value of gemcitabine was observed at 24 h after the administration.(J Intervent Radiol,2007,16: 685-687)