荧光定量PCR技术在小儿尿液巨细胞病毒检测中的应用

目的探讨荧光定量聚合酶链式反应(FQ-PCR)方法检测尿液巨细胞病毒在儿童CMV感染诊断中的意义。方法分别应用FQ-PCR和ELISA法检测75例确诊或高度怀疑为CMV感染和25例对照儿童尿CMV-DNA和血清CMV-IgM,比较病毒DNA与血清抗体的平行性和敏感性。结果75例确诊或疑诊CMV活动性感染患儿中,检测到CMV-DNA 66例;检测到CMV-IgM 54例。两种方法阳性符合率为77%,FQ-PCR和ELISA法阳性检出率分别为88%和72%,有统计学差异(P<0.05)。结论FQ-PCR法检测尿CMV-DNA对诊断活动性CMV感染具有良好的应用价值。

The clinical significance of determination of cytomegalovirus infection in urine of infants by FQ-PCR

Objective To explore the significance of determining of urine cytomegalovirus(CMV) DNA in 20-. -0-diagnosis of cytomegalovirus infection in children by fluorescent quantitative polymerase chain reaction(FQ-PCR) assay. Methods CMV DNA and anti-CMV IgM in the urine of 75 suspected CMV infected children and 25 controls were determined by FQ-PCR and ELISA. Results Out of the 75 children suspected with active CMV infection CMV DNA was detected from 66 children and CMV-IgM was detected from 54 children with the positive rates of 88% and 72%,respectively,showing significant difference(P<0.05).The positive agreement of the two methods was 77%. Conclusion FQ-PCR can be widely used in detection of CMV DNA for diagnosis of active CMV infection in children.