三七总皂苷对oxLDL诱导的人脐静脉内皮细胞CD40, VCAM-1表达的影响

目的    探讨三七总皂苷(TPNS)对氧化型低密度脂蛋白（oxLDL）诱导的人脐静脉内皮细胞（HUVECs）白细胞分化抗原40（CD40）、血管细胞粘附分子-1（VCAM-1）表达的影响。方法    原代培养HUVECs。细胞分为空白组、刺激组、小剂量（100mg/L）TPNS组、大剂量（200mg/L）TPNS组及辛伐他汀组并予相应处理。采用MTT法测定HUVECs活性，实时定量RT-PCR检测VCAM 1的基因表达，Western blot 分析CD40蛋白表达量。结果    TPNS及辛伐他汀均可以升高HUVECs的活性（P＜0.05, P＜0.001），下调HUVECs表达免疫炎症因子CD40 (P＜0.05, P＜0.01)及VCAM-1（P均＜0.001）的水平。且大剂量TPNS作用优于小剂量TPNS（P＜0.05）。结论    TPNS能够减轻oxLDL对内皮细胞的损伤，降低内皮细胞免疫炎症因子的表达，在防治动脉粥样硬化中具有重要作用。

Effect of total panax notoginsenosides on expression of CD40, VCAM-1 induced by oxLDL in human umbilical vein endothelial cells

Objective   To explore the effect of total panax notoginsenosides (TPNS) on expression of cell differentiation antigen 40 (CD40) and vascular cell adhesion molecule-1 (VCAM-1) in human umbilical vein endothelial cells (HUVECs) induced by oxidized low density lipoprotein (oxLDL). Methods    Primary culture HUVECs cells were divided into five groups: the control, the model, the low dose (100mg/L) the TPNS, high dose (200mg/L) TPNS and the Simvastain group. Apart from the control group, cells in other groups were given corresponding treatment. Cytoactivity was measured by MTT.  mRNA expression of VCAM-1 was detected by real time quantitative RT-PCR. Protein expression of CD40 was measured by Western blot. Results    TPNS and Simvastatin could up-regulate the cytoactivity of HUVECs(P＜0.05, P＜0.001),  and down-regulate expression of CD40(P＜0.05, P＜0.01), VCAM-1（P＜0.001）in HUVECs induced by oxLDL. In addition, the effect of high dose TPNS was superior to the low dose TPNS (P＜0.05). Conclusion    TPNS can relieve injury and lower expression of immune inflammatory factors in HUVECs induced by oxLDL. TPNS has a potential effect in preventing and treating atherosclerosis.