| 大豆发酵制品中总异黄酮的含量测定 |
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| 引用本文： | 崔力剑,黄芸,窦玉红,詹文红.大豆发酵制品中总异黄酮的含量测定[J].时珍国医国药,2008,19(2):316-317. |
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| 作者姓名： | 崔力剑 黄芸 窦玉红 詹文红 |
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| 作者单位： | 1. 河北医科大学中医学院,河北,石家庄,050091
2. 河北医科大学药学院,河北,石家庄,050017 |
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| 基金项目： | 河北省科学技术研究与发展计划项目（No.06276471）;河北省卫生厅项目（No.2006191）;河北医科大学青年基金项目（No.0000060） |
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| 摘 要： | 目的建立一种测定大豆发酵制品——淡豆豉中异黄酮类成分含量的紫外分光光度法分析方法。方法以染料木素为对照品，利用染料木素与氢氧化钠产生反应，在271nm波长处有最大吸收点，用紫外分光光度法测定淡豆豉中总异黄酮的含量。结果淡豆豉中总异黄酮的含量为9．884mg／g，加样回收率为99．3％，相对标准偏差为2．1％。结论方法简便、准确、重现性好，可作为测定淡豆豉中总异黄酮的含量的一种手段，适用于淡豆豉及其它豆制品的日常分析和质量控制。
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| 关 键 词： | 大豆 淡豆豉 氢氧化钠 异黄酮 分光光度法 |
| 文章编号： | 1008-0805（2008）02-0316-02 |
| 收稿时间： | 2007-05-10 |
| 修稿时间： | 2007-08-25 |
Quantitative Determination of Total Isoflavones in Semen Sojae Praeparatum by Ultra - Violet Spectrophotometry |
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| CUI Li-jian,HUANG Yun,DOU Yu-hong,ZHAN Wen-hong.Quantitative Determination of Total Isoflavones in Semen Sojae Praeparatum by Ultra - Violet Spectrophotometry[J].Lishizhen Medicine and Materia Medica Research,2008,19(2):316-317. |
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| Authors: | CUI Li-jian HUANG Yun DOU Yu-hong ZHAN Wen-hong |
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| Abstract: | Objective To establish a UV speetrophotometry for determination of isoflavones in Semen Sojae Praeparatum. Methods Genistein was seleeted as the referenee substanee. Based on the reaetion of genistein and NaOH, whieh has the maxium absorption in 271nm wavelength, speetrophotometry method was used for determination of isoflavones in Semen Sojae Praeparatum. Results The total eontent of isoflavones in Semen Sojae Praeparatum extraets was 9. 884mg/g with mean reeovery 99.3%, and the RSD was 2.1%. Conclusion This method is simple,aeeurate and reprodueible,it ean be used for the routine analysis and quality eontrol of the Semen Sojae Praeparatum and funetion food. |
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