组蛋白去甲基化酶KDM5A在人牙髓细胞及成牙本质细胞分化中的表达

目的探讨组蛋白赖氨酸去甲基化酶KDM5A在人牙髓细胞（hDPC）中的表达模式及成牙本质分化诱导对其表达量的影响。方法体外培养hDPC,实时荧光定量聚合酶链反应和Western blot检测第1代至第8代（P1-P8代）hDPC中KDM5A mRNA和蛋白的表达量;免疫荧光检测KDM5A在hDPC中的分布;对P3代细胞进行成牙本质分化诱导,于第7天和第14天分别检测KDM5A mRNA和蛋白的表达水平。结果体外传代培养hDPC中可检测到KDM5A的表达,KDM5A mRNA和蛋白量均呈先增加后减少的趋势;hDPC细胞质及细胞核中均表达KDM5A;成牙本质向分化诱导7和14 d,KDM5A mRNA和蛋白量高于未诱导组细胞,诱导14 d表达量高于诱导7 d（P〈0.05）。结论 hDPC表达KDM5A,矿化诱导可提高KDM5A的表达。

Expression of histone demethylase KDM5A in human dental pulp cells during odontogenic differentiation process

Objective To investigate the expression of KDM5 A in human dental pulp cells during in vitro odontogenic differentiation. Methods Human dental pulp cells（hDPCs） were cultured from human normal tooth pulp. KDM5 A mRNA and protein in hDPCs from passage 1 to passage 8（P1-P8） were detected using real-time quantitative PCR and western blot. Celluar KDM5 A localization in hDPCs was determined by immunofluorescence. HDPCs at passage 3 were cultured in the odontoblastic differentiation induction media for 7 d and 14 d, and the mRNA and protein level of KDM5 A were confirmed by real-time quantitative PCR and western blot. Results Real-time quantitative PCR and western blot showed that mRNA and protein expression of KDM5 A increased at passage 1-2 and decreased at passage 7-8 in hDPCs. KDM5 A existed in both the cytoplasm and the nucleus of the hDPCs.KDM5 A mRNA and protein expression gradually increased during the odontogenic differentiation of the hDPCs（P〈0.05）. Conclusion KDM5 A present in hDPCs. Odontogenic differentiation induction enhance the expression level of KDM5 A.