三角形四肽重复干扰素诱导蛋白2在肺癌中的表达及其临床意义

目的研究三角形四肽重复干扰素诱导蛋白2(interferon-induced protein with tetratricopeptide repeats 2,IFIT2)在肺癌组织中的表达,并分析其表达水平与患者临床病理特征及预后的关系。方法用组织芯片技术和免疫组织化学染色法分别检测90例肺腺癌、78例肺鳞癌组织,以及相应癌旁组织中IFIT2的表达,用Wilcoxon秩和检验比较肺癌及癌旁组织中IFIT2表达水平的差异,χ^2检验分析肺癌组织中IFIT2表达水平与患者临床病理特征的关系,用Kaplan-Meier生存分析法分析IFIT2表达水平与患者预后的关系,拟合Cox模型评价不同指标与患者预后的关系。结果 IFIT2在肺腺癌、肺鳞癌组织中不表达和低表达为主,在癌旁组织中高表达,在癌组织及癌旁组织中的表达差异有统计学意义(P<0.01);IFIT2染色强度与肺腺癌、肺鳞癌患者临床病理特征无相关性(P>0.05);Kaplan-Merier生存分析显示,在肺腺癌中,IFIT2低表达患者总生存期(OS)较IFIT2高表达患者短(HR=2.392,95%CI:1.103~5.186,P=0.027),多因素Cox比例风险模型显示,远处转移(HR=8.033,95%CI:3.664~17.614,P=0.000)可作为肺腺癌患者预后评判的独立风险因素;在肺鳞癌中,IFIT2低表达患者总生存期(OS)较IFIT2高表达患者短(HR=2.907,95%CI:1.118~7.559,P=0.029),多因素Cox比例风险模型显示,淋巴结转移(HR=3.390,95%CI:1.029~11.175,P=0.045)和IFIT2低表达(HR=3.762,95%CI:1.236~11.451,P=0.020)均可作为肺鳞癌患者预后评判的独立风险因素。结论 IFIT2在肺癌组织中下调表达,提示其在肺癌发生发展过程中发挥重要作用,可作为肺癌患者预后评估的重要因素。

Expression of interferon-induced protein with tetratricopeptide repeats 2(IFIT2) in lung cancer tissue and its clinical significance

Objective To investigate the expression of IFIT2(interferon-induced protein with tetratricopeptide repeats 2) in human lung cancer tissue and analyze the relationship between the IFIT2 expression and clinicopathological features and prognosis. Methods Tissue microarray and immunofluorescence staining were used to examine the IFIT2 expression in lung cancer tissue and their adjacent tissues. Wilcoxon rank test was used to compare the IFIT2 expression in lung cancer and corresponding adjacent tissues. The chi-square test was used to analyze the relationship between the IFIT2 expression in lung cancer tissues and clinicopathological features of the patients. Kaplan-Meier survival analysis was performed to analyze the correlation between IFIT2 expression and patients′ overall survival. Cox model was used to analyze the correlation between different clinical parameters and prognosis. Results There was significant difference for the IFIT2 expression between the lung cancer tissues and adjacent tissues(P<0.01). There was no significant correlation between IFIT2 expression and clinicopathological features of patients(P>0.05). In lung adenocarcinoma and squamous cell carcinoma, Kaplan-Merier survival analysis showed that the overall survival(OS) of patients in IFIT2 low expression group was significantly shorter than that in IFIT2 high expression group(HR=2.392, 95%CI: 1.103-5.186, P=0.027;HR=2.907, 95%CI: 1.118-7.559, P=0.029, respectively). Multi-factor Cox model analysis indicated that distant metastasis(HR=8.033, 95% CI: 3.664-17.614, P=0.000) was independent prognostic factors for lung adenocarcinoma, lymph node metastasis(HR=3.390, 95% CI: 1.029-11.175, P=0.045) and IFIT2 low expression(HR=3.762,95%CI: 1.236-11.451, P=0.020) were independent prognostic factors for lung squamous cell carcinoma. Conclusion The down-regulated expression of IFIT2 in lung cancer tissues suggests that it may play an important role in initiation and development of lung cancer. It could be used as a valuable risk factor to predict the prognosis of lung cancer patients.