重复经颅磁刺激对慢性不可预见性应激大鼠抑郁样行为及海马脂质的影响

目的:探讨重复经颅磁刺激(repetitive transcranial magnetic stimulation，rTMS)对慢性不可预见应激(chronic unpredictable stress，CUS)大鼠抑郁样行为及海马脂质的影响。方法:将24只SD大鼠按照随机数字表法分为对照组(sham组)、模型组(CU...

Effects of repetitive transcranial magnetic stimulation on depressive behavior and hippocampal lipid in rats with chronic unpredictable stress

Objective To investigate the effect of repetitive transcranial magnetic stimulation(rTMS)on the hippocampal lipidome in a rat model of chronic unpredictable stress(CUS).Methods Twenty-four SD rats were randomly assigned to the following 3 groups(n=8 for each group):sham group,CUS group and CUS+rTMS group.The sham group received only sham stimulation and rats in the CUS and CUS+rTMS group were subjected to CUS stimulation.Then,rats received 5 Hz rTMS(5 Hz,1.26 Tesla)or sham rTMS for 7 days.After the last stimulation,all rats underwent sucrose preference test,open filed test and forced swimming test so as to observe the effect of rTMS on depressive behavior.Then,rats were sacrificed,and the levels of lipid composition in hippocampus were determined by high performance liquid chromatography mass spectrometry and analyzed by lipid search software version 4.1 and SIMCA-P 14.1.The software of SPSS 19.0 was used for statistical analysis.Univariate analysis of variance was used for comparison among groups,and Tukey test was used for multiple comparison.Results(1)There were significant differences in open field test,sugar preference test and forced swimming test among the three groups(F=6.853-7.466,all P<0.05).In the open field experiment,the exploring time and percentage of movement distance in central area of rats in CUS group((50.72±6.38)s,(11.41±1.55)%)was significantly less than that of sham group((86.06±7.31)s,(18.60±1.21)%)and CUS+rTMS group((79.87±7.87)s,(16.74±1.27)%)(all P<0.05).The results of sucrose preference test showed that the percentage of sucrose intake of rats in CUS group((37.63±6.06)%)was significantly lower than that in sham group((68.30±6.39)%)and CUS+rTMS group((62.68±5.50)%)(both P<0.05).In forced swimming test,the immobility time of rats in CUS group((137.60±13.36)s)was significantly longer than that of sham group((80.57±10.36)s)and CUS+rTMS group((86.14±11.49)s)(both P<0.05).(2)The levels of lipid composition in hippocampus were significantly different in the three groups(F=3.826-15.440,all P<0.05).The contents of phosphatidylethanolamine(PE)((20850±956.56)×10^7,(24133.33±1242.04)×10^7),phosphatidylinositol(PI)((788.78±136.11)×10^7,(953.65±131.26)×10^7),lysophosphatidylcholine(LPC)((340.29±35.66)×10^7,(275.32±35.78)×10^7),creatine phosphate(CerP)((239.65±18.14)×10^7,(293.82±38.28)×10^7),sphingosine(So)((22.96±4.04)×10^7,(15.36±3.87)×10^7),diglyceride(DG)((3.35±0.85)×10^7,(4.57±1.02)×10^7)and monoglyceride(MG)((6.71±0.82)×10^7,(7.94±0.91)×10^7)in hippocampus of rats in CUS group were significantly higher than those of sham group(all P<0.05),while the phosphatidic acid(PA)((424.52±33.38)×10^7,(509.22±42.09)×10^7)and acyl carnitine(AcCa)((2.68±0.33)×10^7,(3.39±0.33)×10^7)decreased(both P<0.05).Compared with CUS group,the contents of PE(21816.67±928.26)×10^7,PI(83.16±91.52)×10^7,LPC(323.59±33.91)×10^7,CerP(236.39±32.02)×10^7,So(23.35±4.46)×10^7,DG(3.16±0.85)×10^7 and MG(7.03±0.26)×10^7 in the hippocampus of CuS+rTMS group decreased,while the contents of PA(421.55±44.28)×10^7 and ACCA(2.56±0.32)×10^7 in the hippocampus of CUS+rTMS group increased(all P<0.05).Conclusion The levels of glycerophospholipids,glyceroglycerides,sphingolipids,fatty acids and other lipids in the hippocampus of CUS model rats are abnormal.And the 5 Hz rTMS intervention can ameliorate the depression like behavior and the disturbances of lipid in hippocampus of CUS model rats.