青少年抑郁症患者代谢组学变化及舍曲林干预的影响

目的:探究青少年抑郁症患者血清中潜在生物标志物，为抑郁症临床诊断及药物作用机制研究提供依据。方法:筛选符合纳入标准的45例青少年抑郁症患者和47例健康对照(HC组)。抑郁症患者使用舍曲林(100 mg/d)治疗4周，治疗前作为DN-MDD组，治疗后作为DT-MDD组。治疗前后样本采用汉密尔顿抑郁评定量表(HAMD)对两...

Metabonomics changes in adolescents with depressive disorder and the effect of sertraline intervention

Objective To detect the potential biomarkers in the serum of adolescents with depressive disorder and provide the basis for clinical diagnosis of depression.Methods A total of 45 adolescents with depression who met the inclusion criteria and 47 healthy controls(HC group)were screened.Patients with depression were treated with sertraline(100 mg/d)for 4 weeks.The patients before sertraline treatment were called DN-MDD group and then were called DT-MDD group after sertraline treatmant.Hamilton Depression Scale(HAMD)was used to evaluate the efficacy of the two groups before and after treatment.The serum was collected for UPLC-MS/MS to determine the changes in profiles of endogenous metabolites.And the software of SPSS 21.0,XCMS and Analyst 3.0 were used to analyze the data.The reliability and feasibility of clinical diagnosis were further analyzed by ROC via GraphPad 8.0.Results After 4 weeks of sertraline treatment,the patients'HAMD score decreased(before:19.71±1.72,after:15.36±1.91)and the effective rate reached 51%(P<0.01).Multivariate statistical analysis showed that HC group and DN-MDD group and DT-MDD group had significant differences in metabolic profile.Compared with healthy controls,11 different metabolites were found in the plasma of DN-MDD group including glycerate,adenosine,creatine and so on.After treatment,concentrations of glucose,glutamate,aspartate,sucrose,creatine,pyruvate and adenosine were significant changes in serum of adolescent depression patients.Area under ROC curve of glucose and glutamic acid were>0.9,and the AUC of aspartic acid,sucrose,creatine,pyruvate and adenosine were>0.7,suggesting that these 7 metabolites had different degrees of diagnostic ability.Conclusion There are different metabolic profiles between adolescents with or without depression,and the antidepressant sertraline is helpful to maintain the level of substance metabolism which can be used as one of the treatment methods for adolescent depression.Our research may support the new strategy for clinical diagnosis and treatment of adolescent depression.