| 石墨烯对氧化应激引起的毛细胞损伤的保护作用研究 |
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| 引用本文： | 董健菲,陆玲,林川耀,刘勇,周函,朱成文,潘明洁,唐明亮,高下. 石墨烯对氧化应激引起的毛细胞损伤的保护作用研究[J]. 中华耳科学杂志, 2021, 0(1): 101-107 |
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| 作者姓名： | 董健菲 陆玲 林川耀 刘勇 周函 朱成文 潘明洁 唐明亮 高下 |
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| 作者单位： | 南京大学医学院附属鼓楼医院耳鼻咽喉头颈外科;南京大学医学院附属鼓楼医院耳鼻咽喉研究所;南京大学医学院附属鼓楼医院中心实验室;东南大学生命科学院 |
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| 基金项目： | 国家自然科学基金青年基金(81700913);江苏省科教强卫工程江苏省青年医学人才基金(QNRC2016002)。 |
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| 摘 要： | 目的 通过建立由H2O2诱导的HEI-OC1细胞氧化应激模型,验证石墨烯对氧化应激后毛细胞的保护作用,并探究石墨烯减轻毛细胞氧化应激损伤的可能机制.方法 我们将二维石墨烯材料(2D-G)作为实验组,TC玻片(TCPs)作为对照组.首先用不同浓度的H2O2处理接种于两种材料上的HEI-OC1细胞,分别作用4h和12h后,...
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| 关 键 词： | 石墨烯 氧化应激 毛细胞损伤 毛细胞保护 |
Graphene Inhibits Damage to Hair Cells by Oxidative Stress |
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| DONG Jianfei,LU Ling,LIN Chuanyao,LIU Yong,ZHOU Han,ZHU Chengwen,PAN Mingjie,TANG Mingliang,GAO Xia. Graphene Inhibits Damage to Hair Cells by Oxidative Stress[J]. Chinese Journal of Otology, 2021, 0(1): 101-107 |
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| Authors: | DONG Jianfei LU Ling LIN Chuanyao LIU Yong ZHOU Han ZHU Chengwen PAN Mingjie TANG Mingliang GAO Xia |
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| Affiliation: | (Department of Otolaryngology-Head and Neck Surgery,Nanjing Drum Tower Hospital of Nanjing University School of Medicine,Nanjing 210008;Research Institute of Otolaryngology,Nanjing 210008;Central Laboratory of Nanjing Drum Tower Hospital of Nanjing University School of Medicine,Nanjing 210008;College of Life Science,Southeast University,Nanjing,210096) |
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| Abstract: | Objective To verify the protection of the hair cells by 2D-graphene against oxidative stress damage by H2O2 and possible mechanisms.Methods HEI-OC1 cells were treated with 2D-G(study group)or TCPs(control group)and exposed to different concentrations of H2O2.Cell survival was examined by the CCK 8 method and immunofluorescence staining of Myosin7a(a hair-cell-specific protein)after 4 hours and 12 hours of H2O2 exposure and compared between the two groups.Whole ROS level in cells and ROS level in mitochondria were determined by detection of DCFHDA and Mito-sox using fluorescent probes.Changes of ROS levels and levels of apoptosis after oxidative stress were examined by flow analysis and immunofluorescence and by TUNNEL assay,respectively,and compared between the two groups.Results The rate of HEI-OC1 cells survival was greater after oxidative stress,with significantly reduced intracellular and mitochondrial ROS levels and cells apoptosis when treated with graphene.Conclusion Graphene can significantly reduce the level of ROS in cytoplasm and mitochondria in cochlear hair cell line HEI-OC1 cells at risk of oxidative stress damage,thereby inhibiting apoptosis and protecting the cells from oxidative stress. |
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| Keywords: | Graphene Oxidative stress Hair cell injury Hair cell protection |
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