应用AB-8大孔树脂纯化新乌头碱和乌头总碱

目的：对影响AB-8树脂分离纯化乌头总生物碱和新乌头碱的各种因素进行研究，优化分离工艺。方法：以川乌总碱和新乌头碱的含量为指标，考察AB-8型大孔树脂分离纯化乌头总生物碱和新乌头碱的最佳吸附及洗脱条件。结果：AB-8树脂纯化乌头总生物碱和新乌头碱的最佳工艺为上样液浓度1g生药／mL，药液pH10，吸附时间为6h，7倍体积95％乙醇洗脱效果好。AB-8树脂可重复使用5次。经AB-8树脂处理后乌头总碱提取率可达80％以上，终产品总生物碱纯度可达30％以上，结论：AB-8树脂可用于川乌总碱的工业化生产。

Study on Separation and Purification of Total Alkaloids and Mesaconine with AB-8 Macroporous Resin

ATM: To systematically study the factors which affect separation and purification of the total alkaloids and mesaconine and optimize the purification technology.METHODS: The optimal conditions of absorption and elution were studied in the process of purifying two indexes with AB-8 macroporous resin by taking the content of the total alkaloids and mesaconine as indexes. RESULTS: AB-8 macroporous resin had the best separating efficiency when the mesaconine content in the extracted solution was 0.2 mg per 1 mL equivalent 1 g raw material, pH of the extracted solution was 10.0; the extracted solution was absorbed for 6 hours; then the resin was eluted using 7 times as much 95% ethanol as the volume of resin; AB-8 macroporous resin could used be five times repeatedly. The rate of extraction and purity of the total alkaloid was 80% and 30% or so respectively after the purification with AB-8 macroporous resin. CONCLUSION: AB-8 can meet industrial requirement.