醛固酮对3T3-L1前脂肪细胞与脂肪细胞内脂素表达和分泌的影响

目的:探讨醛固酮对3T3-L1前脂肪细胞和脂肪细胞内脂素基因表达和蛋白分泌的影响。方法:10-8和10-6mol/L醛固酮加或不加10-6mol/L安体舒通分别干预3T3-L1前脂肪细胞和脂肪细胞24h和48h,用实时RT-PCR测定内脂素和盐皮质激素受体(MR)mRNA的表达,酶联免疫法测定培养液中内脂素的浓度。结果:醛固酮作用于3T3-L1前脂肪细胞,内脂素mRNA表达减少,培养液中蛋白浓度变化不明显,MR mRNA表达增高。醛固酮作用于脂肪细胞,内脂素mRNA表达和蛋白浓度均降低,MR mRNA表达增高。安体舒通在一定程度上可对抗醛固酮对内脂素的抑制作用。结论:醛固酮抑制3T3-L1脂肪细胞内脂素的基因表达和分泌。

Visfatin gene expression and secretion are regulated by aldosterone in 3T3 -L1 preadipocytes and adipocytes

AIM: To explore the effect of aldosterone on visfatin gene expression and secretion in 3T3-L1 preadipocytes or adipocytes. METHODS: Aldosterone at concentration of 10-8 or 10-6 mol/L with or without 10-6 mol/L spironolactone was added to cultured 3T3-L1 preadipocytes or adipocytes for 24 h or 48 h. The mRNA levels of visfatin and mineralocorticoid receptor were measured using real time PCR. The concentration of visfatin in the culture medium was determined by ELISA. RESULTS: In 3T3-L1 preadipocytes treated with aldosterone, the mRNA expression of visfatin reduced and the mRNA expression of mineralocorticoid receptor(MR) increased, but the concentration of visfatin in culture medium was not regulated significantly by aldosterone. In adipocytes with aldosterone treatment, the mRNA expression of visfatin and visfatin concentration in culture medium reduced, and mRNA expression of MR increased. The effect of aldosterone was blocked by spironolactone to some extent. CONCLUSION: Aldosterone inhibits the gene expression and protein secretion of visfatin in 3T3-L1 adipocytes.