Functional Identification of a Novel Transport System for Endogenous and Synthetic Opioid Peptides in the Rabbit Conjunctival Epithelial Cell Line CJVE |
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Authors: | Sudha Ananth Senthil Karunakaran Pamela M. Martin Chandrasekharam N. Nagineni John J. Hooks Sylvia B. Smith Puttur D. Prasad Vadivel Ganapathy |
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Affiliation: | (1) Department of Biochemistry and Molecular Biology, Medical College of Georgia, Augusta, Georgia 30912, USA;(2) Laboratory of Immunology, National Eye Institute, Bethesda, Maryland 20892, USA;(3) Department of Cellular Biology and Anatomy, Medical College of Georgia, Augusta, Georgia 30912, USA |
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Abstract: | Purpose To investigate whether conjunctival epithelial cells express transport processes for opioid peptides. Methods We monitored the uptake of [3H]deltorphin II and [3H]DADLE, two hydrolysis-resistant synthetic opioid peptides, in the rabbit conjunctival epithelial cell line CJVE and elucidated the characteristics of the uptake process. Results CJVE cells express robust uptake activity for deltorphin II and DADLE. Both opioid peptides compete with each other for transport. Several endogenous and synthetic opioid peptides, but not non-peptide opioid antagonists, are recognized by the transport process. Though various peptides inhibit the uptake of deltorphin II and DADLE in a similar manner, the uptake of deltorphin II is partly Na+-dependent whereas that of DADLE mostly Na+-independent. The transport process shows high affinity for many endogenous/synthetic opioid peptides. Functional features reveal that this transport process may be distinct from the opioid peptide transport system described in the retinal pigment epithelial cell line ARPE-19 and also from the organic anion transporting polypeptides, which are known to transport opioid peptides. Conclusions CJVE cells express a novel, hitherto unknown transport process for endogenous/synthetic opioid peptides. This new transport process may offer an effective delivery route for opioid peptide drugs to the posterior segment of the eye. |
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Keywords: | conjunctival epithelial cell non-peptide opioid antagonists opioid peptides organic anion transporting polypeptides transport process |
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