银杏黄酮苷元对人脐静脉内皮细胞ECV304增殖的影响

目的：体外观察银杏黄酮苷元（GA）对人脐静脉内皮细胞ECV304增殖作用的影响,初步探讨其对内皮细胞的保护作用。方法：分别设对照组、空白组和实验组;实验组设GA6.25、12.5、25、50、100、500 mg/L共6个浓度孵育ECV304 48 h,25 mg/LGA与ECV304共同孵育6、12、24及48 h共4个时间点,采用MTT比色法观察GA对人脐静脉内皮细胞ECV304增殖的影响。结果：6.25～50 mg/L GA与ECV304共同培养48 h,细胞形态基本正常,细胞增殖率均大于100%,与对照组（未加药）比较差异显著（P〈0.05）,且各组细胞增殖率随药物浓度增加而增加,P〈0.05;GA25 mg/L和脐静脉内皮细胞ECV304共培养6～24 h,各组细胞增殖率随作用时间延长而增加,各组间比较差异显著（P〈0.05）。结论：银杏黄酮苷元促进人脐静脉内皮细胞ECV304增殖的作用具有时间和浓度效应,提示GA具有保护血管内皮细胞的作用。

Effects of Ginkgetin Aglycone on Proliferation of Human Umbilical Venous Endothelial Cell Line, ECV304

Objective：To investigate the effects of ginkgetin aglycone（GA） on the proliferation of human umbilical venous endothelial cells,ECV304,and so as to explore the feasibility of GA in protecting vessel endothelial cells.Methods： ECV304 were cultured with GA of different concentrations for different hours.Cell proliferation rates were determined with MTT assay.Results： ECV304 cultured with GA of 50～6.25 mg/L for 6 to 48 hours were normal morphologically.Cell proliferation rates of different drug-groups all exceeded 100%,which was different from that of control group significantly（P0.05）.Proliferation rates of ECV304 cultured with GA of 25～6.25mg/L for 6 to 24 hours increased along with the increase of GA＇s concentration and action time to some extend.Differences among the groups were significant（P0.05）.Conclusions： GA promotes proliferation of ECV304 in a dose and time dependant manner.GA may have some protective effect on vessel endothelial cells.