| 高效液相色谱法测定滴霉洗剂中蛇床子素的含量 |
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| 引用本文： | 伏东宁,凌娅,陈艳明.高效液相色谱法测定滴霉洗剂中蛇床子素的含量[J].中国生化药物杂志,2001,22(6):303-304. |
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| 作者姓名： | 伏东宁 凌娅 陈艳明 |
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| 作者单位： | 1. 连云港市中医院
2. 江苏康缘股份有限公司,江苏,连云港,222001 |
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| 摘 要： | 目的研究滴霉洗剂中蛇床子素含量测定的方法。方法采用高效液相色谱法以甲醇 水 (75∶2 5 )为流动相 ,色谱柱为AlltimaC18(2 5 0mm× 4.6mm ,5 μm) ;流速为 1ml/min ;检测波长为 32 2nm。结果线性范围为 6 .0～18.0 μg ,r =0 .9999,加样回收率为 98.99% ,RSD为 0 .86 % (n =6 )。结论此法简便易行 ,结果准确 ,重现性好 ,灵敏度高 ,可用于控制生药及制剂的质量
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| 关 键 词： | 高效液相色谱法 蛇床子素 含量 |
| 文章编号： | 1005-1678(2001)06-0303-02 |
| 修稿时间： | 2001年5月25日 |
Determination of osthol in lotion Dimei by HPLC |
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| FU Dong ning ,LING Ya ,CHEN Yan ming.Determination of osthol in lotion Dimei by HPLC[J].Chinese Journal of Biochemical Pharmaceutics,2001,22(6):303-304. |
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| Authors: | FU Dong ning LING Ya CHEN Yan ming |
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| Institution: | FU Dong ning 1,LING Ya 2,CHEN Yan ming 2 |
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| Abstract: | PurposeThe aim is to investigate a method of determination of Osthol in Lotion Dimei. Methods HPLC method was used with methanol water(75:25)as the mobile phase. The chromatographic column was Alltima C 18 (250 mm×4.6 mm,5 μm),the flow rate was 1.0 ml/min,and the detection wave length was 322 nm.ResultsThe linear range was 6.0～18.0 μg, r =0.999 9,marked recovery rate was 98.99%, and RSD =0.86%( n =6).ConclusionThis method is simple , easy and reliable.It also has a good repeatability and sensibility. |
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| Keywords: | HPLC Osthol Determination |
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