| 阳离子脂质体介导HSV-TK/ACV系统对人脑胶质瘤细胞增殖活性的影响 |
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| 引用本文： | 苏君,杨海城,肖宏,张学新. 阳离子脂质体介导HSV-TK/ACV系统对人脑胶质瘤细胞增殖活性的影响[J]. 肿瘤防治研究, 2002, 29(2): 83-86 |
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| 作者姓名： | 苏君 杨海城 肖宏 张学新 |
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| 作者单位： | 150040,哈尔滨医科大学附属第三医院神经外科 |
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| 基金项目： | 黑龙江省自然科学资金资助项目(D0 0 33) |
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| 摘 要： | 目的 探讨HSV TK/ACV系统对人脑胶质瘤细胞增殖活性的影响。方法 用阳离子脂质体Lipofectamine将 pCR3 Uni及含HSV TK基因的真核表达载体 pCR3 TK转染至人脑胶质瘤细胞株TJ90 5中 ,筛选出阳性克隆 ,阳性细胞克隆给予ACV(5 0mg/ml) ,72小时后 ,收集玻片 ,进行AgNOR染色。 结果 转染HSV TK基因的细胞 ,在给予ACV后 ,细胞增殖活性明显降低 ,转染 pCR3 Uni和 pCR3 |TK细胞克隆的AgNOR颗粒数分别为 1 4.3 3和 6.67(P <0 .0 1 )。结论 AgNOR计数是一种操作简便、检测细胞增殖活性的方法 ,为研究HSV TK/ACV系统的抗肿瘤机制提供帮助
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| 关 键 词： | Lipofectamine HSV/ACV系统 胶质瘤 AgNOR 增殖活性 |
| 文章编号： | 1000-8578(2002)02-0085-02 |
| 收稿时间： | 2001-04-10 |
| 修稿时间： | 2001-04-10 |
Effects of the proliferation activity of human glioma cells with HSV-TK/ACV system mediated by cationic liposome |
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| SU Jun,YANG Hai|cheng,XIAO Hong,et al. Effects of the proliferation activity of human glioma cells with HSV-TK/ACV system mediated by cationic liposome[J]. Cancer Research on Prevention and Treatment, 2002, 29(2): 83-86 |
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| Authors: | SU Jun YANG Hai|cheng XIAO Hong et al |
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| Affiliation: | SU Jun,YANG Hai|cheng,XIAO Hong,et al Department of Neurosurgery,Third Hospital of Harbin Medical University,Harbin 150040,China |
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| Abstract: | Objective To study the effect of the proliferation activity of human glioma cells with HSV|TK/ACV system.Methods The eukaryotic expressing vectors pCR3|TK containing the HSV|TK gene and pCR3|Uni were transferred into TJ905 cells with cationic liposome, Lipofectamine .After transfection,G418 was used to select the positive clones. The psoitive clones were given ACV(50μg/ml) and collected afer 72 hours. The glass slides were stained with silver.Results The number of silver stained AgNORs in the cell cl... |
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| Keywords: | Lipofectamine HSV|TK/ACV Glioma AgNOR Proliferation activity |
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