正交设计法优选酸枣仁皂苷的大孔吸附树脂纯化工艺

目的:比较不同型号大孔吸附树脂对酸枣仁皂苷的纯化效果,并优选其纯化工艺.方法:以酸枣仁皂苷A为指标成分,HPLC-ELSD测定指标成分含量.通过静态吸附法和动态吸附法优选大孔树脂型号,采用正交试验和单因素试验考察其纯化工艺.结果:酸枣仁皂苷A在0.216 ～2.16μg与峰面积成良好线性关系,平均加样回收率为95.77％,RSD 0.71％.ADS-7型大孔吸附树脂能有效除去色素,纯化效果最好,其优化纯化工艺为柱高径比3∶1,药液质量浓度1.0 g·mL-1,吸附速度1.0 BV·h-1,树脂生药吸附量0.4 g·g-1,洗脱溶媒70％乙醇,洗脱速度2 BV·h-1,洗脱溶媒用量4 BV.结论:ADS-7型大孔吸附树脂对酸枣仁皂苷A纯化效果较好,工艺稳定可行,可用于工业化生产.

Optimization of Purification Technology for Saponins from Ziziphus jujube with Macroporous Adsorption Resin by Orthogonal Test

Objective:To compare purification effect of saponins from Ziziphus jujube with different types of macroporous adsorption resin,and to optimize its purification technology.Method: With jujuboside A as index,the content of it was determined by HPLC-ELSD.Types of macroporous resin were optimized by static adsorption and dynamic adsorption method,purification technology of it was investigated by orthogonal test and single factors test.Result: For jujuboside A,there was a good linear relationship in the range of 0.216-2.16 μg,average recovery was 95.77%,RSD was 0.71%.ADS-7 macroporous resin could effectively remove pigment with better purification effect of total saponins than other resins,optimum technological parameters were:column height-diameter ratio of 3∶1,the concentration of liquid 1.0 g·mL-1,absorption velocity 1.0 BV·h-1,resin adsorption quantity 0.4 g·g-1,elution solvent 70% ethanol,elution speed 2 BV·h-1,elution solvent volume 4 BV.Conclusion: ADS-7 macroporous resin had a good purification effect on jujuboside A,optimized technology was stable,feasible,it could be used for industrial production.