山楂三萜酸类有效部位提取纯化工艺比较

目的:初步确定山楂中三萜酸类有效部位提取纯化工艺.方法:比较三萜酸类有效部位的4种提取方法和3种纯化方法,以三萜酸类成分含量为考察指标进行提取纯化工艺试验研究.结果:采用碱醇水浸提法时三萜酸类成分提取效率高,基本提取完全.活性碳吸附结晶纯化法产物中三萜酸类成分纯度＞70％.结论:优选的碱醇水浸提法和活性碳吸附结晶纯化法高效、简便,易于规模化,适宜作为山楂中三萜酸类有效部位提取纯化工艺.

Comparison of Extraction and Purification Technology for Triterpenoid Acids Effective Part from Crataegus pinnatifida

Objective: To preliminary determine extraction and purification process of triterpenoid acids effective part from Crataegus pinnatifida. Method: Four extraction methods and three purification methods of triterpenoid acids effective part were compared,extraction and purification technology test was investigated with the content of triterpenoid acids as index. Result: Extraction efficiency of triterpenoid acids was optimum by alkaline alcohol water extraction method,triterpenoid acids were basic extracted completely.Purity of triterpenoid acids was more than 70% by activated carbon adsorption crystallization purification method. Conclusion: Optimized extraction and purification method was efficient, simple, easy to large scale, they were suitable for extraction and purification process of triterpenoid acids effective part from C. pinnatifida.