当归补血汤表征参数与其补气功效的关系初探

目的:研究当归补血汤理化表征参数与其补气功效的关系,探索利用理化参数表征中药汤剂质量的可行性.方法:采用4种提取工艺(8倍量水提取1次,每次1 h;8倍量水提取3次,每次2 h;10倍量水提取3次,每次1.5 h;12倍量水提取3次,每次1h)制备当归补血汤,测定其25℃时的表面张力、pH、电导率和渗透压；将224只小鼠随机分为正常组、模型组、阳性组和当归补血汤1,2,3,4号组,每组32只；模型组、阳性组、当归补血汤组每天上午ig施以100％大黄煎剂0.5 mL/只,下午强制游泳5 min,造模持续8d;于第9天开始给药,当归补血汤各组小鼠ig给予当归补血汤10 g·kg-1·d-1,阳性组给予等量的复方阿胶浆,正常组与模型组小鼠给予等容量生理盐水,连续给药7d.于末次给药1.5h后测定小鼠的体温,抗疲劳、耐缺氧、耐寒时间和肝脏、脾脏、胸腺指数；最后,关联理化参数、药效和主要活性组分含量,并对试验数据进行统计和回归分析.结果:经多项式回归分析,表面张力、pH、电导率、渗透压与组分含量和药效的综合评分之和的相关系数分别为0.864 8,0.9994,0.973 8,0.937 0,说明当归补血汤的理化表征参数、组分含量和补气药效三者的相关性较为显著.结论:当归补血汤理化参数与其补气功效相关性较强,初步表明可利用上述特征参数来表征当归补血汤体系的内在质量.

Preliminary Studies on Relationship between Physical and Chemical Parameters and Effect of Invigorating Qi by Danggui Buxue Decoction

Objective:To study the relationship between physical and chemical parameters and effect of invigorating Qi by Danggui Buxue decoction,and to explore the feasibility of characterizing the quality of decoction by physical and chemical parameters.Method: Danggui Buxue decoction was prepared by four methods(extracting once with 8 times water for 1 h;extracting three times with 8 times water for 2 h;extracting three times with 10 times water for 1.5 h;extracting three times with 12 times water for 1 h),the physical and chemical parameters of which such as surface tension,pH value,conductivity and osmotic pressure were determined at 25 ℃.224 mice were randomly divided into 7 groups,with 32 in each group.model group,E Jiao compound group and Danggui Buxue decoction groups were treated with 100% Rhubarb decoction every morning and forced to swim for 5 min every afternoon for eight days.Danggui Buxue decoction groups,E Jiao compound group,blank group and model group were treated with Danggui Buxue decoction,E Jiao compound,normal saline respectively from 9th to 15th day.Temperature,time for resistance to fatigue,hypoxia tolerance and the hardy,liver,spleen and thymus index were determined at 1.5 h after the last administration.Finally,relationships between physical and chemical parameters,effect and the content of main active ingredients of Danggui Buxue decoction were studied,and mathematical statistical analysis and regression analysis were used to analyze these data.Result: Through polynomial regression analysis,correlation coefficients between surface tension,pH value,conductivity,osmotic pressure,and the score of component content and effect were respectively 0.864 8,0.999 4,0.973 8,0.937 0.It showed that physical and chemical parameters closely correlated with component content and effect of invigorating Qi.Conclusion: Physical and chemical parameters have a good correlation with effect of invigorating Qi of Danggui Buxue decoction.These results preliminarily suggest that such parameters could be used to characterize the quality of decoction.