HPLC同时测定白虎汤中4种有效成分的含量

目的:建立白虎汤中4种有效成分含量的同时测定方法.方法:利用HPLC同时测定白虎汤中新芒果苷、芒果苷、甘草苷和甘草酸铵的含量,Alltima C18色谱柱(4.6 mm × 250 mm,5μm),流动相为乙腈-25 mmol· L-的KH2PO4溶液,梯度洗脱,检测波长为256 nm,柱温25℃,流速1 mL· min-1.结果:新芒果苷、芒果苷、甘草苷和甘草酸铵的线性范围分别为0.15～3.00 μg(r =0.999 9),0.10-2.00 μg(r=0.999 9),0.04～0.80 μg(r =0.999 9),0.04～0.80 μg(r =0.999 9),平均加样回收率在95.0％ ～ 104.5％.结论:该方法方便、稳定、可靠,适用于白虎汤的质量控制.

Simultaneous Determination of Four Active Components in Baihu Decoction by HPLC

Objective:To establish the method for simultaneous determination of four active components in Baihu Decoction.Method: Simultaneous determination of neomangiferin,mangiferin,liquiritin and ammonium glycyrrhizinate in Baihu Decoction by HPLC.An Alltima C18(4.6 mm×250 mm,5 μm) column was used,the mobile phase was acetonitrile and 25 mmol·L-1 of KH2PO4 solution in gradient elution,the detection wavelength was set at 256 nm,the column temperature was kept at 25 ℃ and the flow rate was 1 mL·min-1.Result: The linear range of neomangiferin,mangiferin,liquiritin and ammonium glycyrrhizinate was 0.15-3.00 μg(r=0.999 9),0.10-2.00 μg(r=0.999 9),0.04-0.80 μg(r=0.999 9),0.04-0.80μg(r=0.999 9) separately.The average recovery was between 95.0%-104.5% for four ingredients.Conclusion: The method is convenient,stable,reliable and suitable for quality control of Baihu decoction.